Enzymes. Characteristics of Enzymes. Proteins Monomer is: _______ ______ Catalysts Start or speed up chemical reactions without being used up. In case you wondered…How do enzymes work?. Lower Activation Energy to speed up rates of reaction
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Enzyme + Substrate ES complex Enzyme + Products
Enzymes can be used to break down molecules
Enzymes can also be used to
bond two substrates into one product
In this lab- there are three reagents:
Which is the enzyme?
Which is the substrate?
What is the other reagent then?!?
What kind of reaction is being started in this reaction (breaking down or building up?)
What are the products of this reaction?
H2O2 H20 + O
How will we know if the reaction occurred?
H2O2 H20 + O
Guiacol turns brown when oxidized. (and it gets more and more brown as more of the guiacol is oxidized).
How do we quantify “how brown” it is?
With a spectrophotometer!
Results of the lab (graph)
Tannic acid is used to form a scab on a burn. An egg becomes hard boiled when placed in hot water. What is similar about these two events?
Acid and heat cause a denaturation of protein. They both break bonds in the structure of protein.
If the temperature gets too high, the enzyme may be denatured!