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EMB agar. Only gram-negative bacteria grow on EMB agar. (Gram-positive bacteria are inhibited by the dyes eosin and methylene blue added to the agar.) Based on its rate of lactose fermentation, E. coli produces dark, blue-black colonies with a metallic green sheen on EMB agar.

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emb agar
EMB agar

Only gram-negative bacteria grow on EMB agar. (Gram-positive bacteria are inhibited by the dyes eosin and methylene blue added to the agar.) Based on its rate of lactose fermentation, E. coli produces dark, blue-black colonies with a metallic green sheen on EMB agar.

growing on emb agar
Growing on EMB Agar

Enterobacter aerogenes

nutrient agar
Nutrient Agar

Micrococcus luteus

Pseudomonas aeruginosa

mannitol salt agar
Mannitol Salt Agar

Mannitol salt agar is both a selective and differential growth medium. It is used to differentiate pathogenic Staphylococcusspecies from nonpathogenic members of the genus Micrococcus.

Fermentation of mannitol: This medium contains an indicator, phenol red. The indicator is a pinkish-red at neutral pH, is really red at ph 7.4 and above (basic) and is yellow below pH 6.8 (acidic). An organism can either ferment (metabolize) mannitol or not and this will be indicated by a color change on the MSA plate--which makes this a differential test.

slide5

 2. S. epidermidisSalt tolerance + Mannitol fermentation -

 1. S. aureusSalt tolerance +Mannitol fermentation + (yellow)

 2. M. luteusSalt tolerance -Mannitol fermentation -

slide6

Staphylococcus aureus Growing on Mannitol Salt Agar.

Staphylococcus epidermidis Growing on Mannitol Salt Agar

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