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Antigen Presentation Fundamentals I for Dentistry & Optometry

Textbook: Medical Microbiology, 6 th Edition, Murray Chapter 11 Jim Collawn, MCLM 350 jcollawn@uab.edu 934-1002. Antigen Presentation Fundamentals I for Dentistry & Optometry. Compare and contrast class I and class II MHC-restricted responses with regard to A. source of antigens

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Antigen Presentation Fundamentals I for Dentistry & Optometry

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  1. Textbook: Medical Microbiology, 6th Edition, Murray Chapter 11 Jim Collawn, MCLM 350 jcollawn@uab.edu 934-1002 Antigen PresentationFundamentals I for Dentistry & Optometry

  2. Compare and contrast class I and class II MHC-restricted responses with regard to • A. source of antigens • B. antigen processing requirements • C. role of chaperones • D. types of T cell involved • 2. Discuss the invariant chain’s role in the demarcation between class I and class II MHC-restricted responses. • 3. Discuss HLA-DM’s possible role in antigen processing. Study Objectives

  3. Humoral and Cell-mediated Immune Responses

  4. Major Players in T cell Responses

  5. T lymphocyte and Macrophage (right)

  6. Cytotoxic T cells (CTLs) • Kill virally infected cells • Kill cells containing cytosolic bacteria • Kill tumor cells • Inflammatory T cells (TH1) • A. Activate macrophages to kill intracellular bacteria • Helper T cells (TH2) • Activate B cells to make antibody Types of T cells

  7. Schematic Diagrams of MHC class I and class II Molecules

  8. MHC class I (top view)

  9. Major Histocompatibility Complex

  10. HLA-DR1 (blue) and HLA-A2

  11. MHC class I

  12. MHC class II

  13. Anchor Residues for MHC class I peptides

  14. Conformation of Peptides Bound to MHC class I

  15. Solvent-accessible Area of H-2Kb

  16. Anchor Residues for MHC class II peptides

  17. Antigen Processing is Necessary for Helper T cell Activation

  18. Antigen-presenting Cells(MHC Class II-positive cells)HLA-DR, HLA-DP, HLA-DQ

  19. Target Cells(MHC Class I-positive cells)HLA-A, HLA-B, HLA-CAll nucleated cells

  20. Degradation of Intracellular Proteins

  21. Transporter associated with Antigen Processing

  22. Generation of Peptide-class I MHC Complexes

  23. Demarcation between MHC class I and class II Processing Pathways

  24. Cytosolic and Endocytic Pathways for Antigen Processing

  25. Assembly of MHC class II Molecules

  26. Compare and contrast class I and class II MHC-restricted • responses with regard to • A. source of antigens • B. antigen processing requirements • C. role of chaperones • D. types of T cell involved • 2. Discuss the invariant chain’s role in the demarcation between class I and class II MHC-restricted responses. • 3. Discuss HLA-DM’s possible role in antigen processing. Study Objectives

  27. Sample Questions • The MHC class II molecule • is associated with ß-2 microglobulin. • binds peptide antigens from the endogenous pathway. • is associated with the invariant chain. • is expressed on all cell types. • Exogenous antigens • are taken up by cells through endocytosis or phagocytosis. • are bound to MHC class I molecules. • are processed by the proteasome. • become associated with MHC class I molecules in the endoplasmic reticulum.

  28. Sample Questions • HLA-DR • presents antigens to cytotoxic T cells. • is a chaperone that facilitates CLIP removal and peptide binding. • is an MHC class II molecule. • binds to endogenous antigens. • The proteolytic activity necessary for the generation of MHC class II-associated peptides is provided by • the proteasome. • the lysosome. • furin. • the endoplasmic reticulum.

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