Isolation & identification of Novel microorganisms for the Treatment of Raw Crustacean Waste to Prod...
Download
1 / 14

Isolation & identification of Novel microorganisms for the Treatment of Raw Crustacean Waste to Produce Chitinous Mater - PowerPoint PPT Presentation


  • 137 Views
  • Uploaded on

Isolation & identification of Novel microorganisms for the Treatment of Raw Crustacean Waste to Produce Chitinous Materials. Carla Harkin. Dr Wolfram Brück Dr Catherine Lynch Dr Denis McCrudden. ChiBio: Work Package 2 Aims.

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about ' Isolation & identification of Novel microorganisms for the Treatment of Raw Crustacean Waste to Produce Chitinous Mater' - lyn


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript

Isolation & identification of Novel microorganisms for the Treatment of Raw Crustacean Waste to Produce Chitinous Materials

Carla Harkin

Dr Wolfram Brück

Dr Catherine Lynch

Dr Denis McCrudden


Chibio work package 2 aims
ChiBio: Work Package 2 Aims Treatment of Raw Crustacean Waste to Produce Chitinous Materials

  • Development of efficient, economical and environmentally-friendly pre-treatment of crustacean shell wastes to extract chitinous material(Raw material sources: Europe/Asia/Africa).

  • Stabilisation of chitinous material for transfer to project partners (UMB, INS, TUM, FhG).

  • Development of assays for constant quality control.


Pre treatment overview
Pre-treatment overview Treatment of Raw Crustacean Waste to Produce Chitinous Materials

Raw material Prep

Optimisation of chemical chitin extraction methods

Identification of novel microorganisms

Optimisation of microbial chitin extraction methods

QC

Up-scaling


Raw material preparation
Raw Material preparation Treatment of Raw Crustacean Waste to Produce Chitinous Materials


Chemical pre treatment
Chemical Pre-treatment Treatment of Raw Crustacean Waste to Produce Chitinous Materials

7. QC Analysis


Microbial pre treatment
Microbial Pre-treatment Treatment of Raw Crustacean Waste to Produce Chitinous Materials

  • Chemical extraction methods- costly and a hazard to the environment.

  • Biological extraction methods- more cost effective while posing less cause for environmental concern.

  • Microbial chitin extraction methods:

    • Direct use of enzymes (Brück et al, 2011; Jo et al, 2011).

    • Fermentation (Jung et al, 2005; Jung et al, 2007; Rao et al, 2000).

      Fermentation

  • Initial optimisation, adapting methods from literature.

  • Identification of novel microorganisms for fermentation.


Fermentation process
Fermentation Process Treatment of Raw Crustacean Waste to Produce Chitinous Materials

Raw material

+

Inoculum

Shaking Incubator

QC

Shaking Incubator

Harvesting & drying

Change of Inoculum

(Jung et al., 2005; Jung et al., 2007a,b; Oh et al., 2007)


Isolation of novel microorganisms
Isolation of Novel Microorganisms Treatment of Raw Crustacean Waste to Produce Chitinous Materials

Isolation from soil samples

Screening for useful properties

Small scale fermentation

Identification

Large scale fermentation

Characterisation


Initial screening
Initial Screening Treatment of Raw Crustacean Waste to Produce Chitinous Materials

  • Demineralisation–acid production.

    • Replicated fermentation method on micro scale.

  • Deproteinisation–protease production.

    • Casein agar plates.

    • Colorimetric protease assay to quantify activity

      (Vishwanatha et al, 2009).

Fig 1: pH indication after addition of Methyl Red Indicator. L-r: negative yellow, negative orange, positive intense red.

Fig 2: Produciton of zone of proteolysis in casein agar.


Selection of organisms
Selection of Organisms Treatment of Raw Crustacean Waste to Produce Chitinous Materials


To be done
To be done Treatment of Raw Crustacean Waste to Produce Chitinous Materials

  • Identification of isolates; PCR, sequencing- BLAST

  • Characterization of isolates: biochemical tests, staining etc.

  • Continue to meet chitinous material production targets.

  • Continuation of fermentation experiments using raw crustacean waste from Ireland, Indonesia and Tunisia.


Future prospects
Future prospects Treatment of Raw Crustacean Waste to Produce Chitinous Materials

  • With select cultures, the aim is to scale-up fermentations to pilot scale to investigate the reality of industrial large scale fermentations in the breakdown of crustacean waste.

  • Development of an economic, environmentally friendly, waste reducing facility.

  • Profit-generating business opportunities for Irish Seafood Industry.


References
References Treatment of Raw Crustacean Waste to Produce Chitinous Materials

  • Brück, W., M., Slater, J. W., Carney, B. F., 2011. Chitin and chitosan from marine organisms. Chitin, Chitosan, Oligosaccharides and their derivatives. Boca Raton: CRC Press.

  • Vishwanatha, T., Spoorthi, N., Reena, V., Divyashree B.C., Siddalingeshwara K.G., Karthic, J., Sudipta K.M., 2010. Screening of substrates for protease production from Bacillus lichenformis. International Journal of Engineering Science and Technology(2)11, pp. 6550-6554

  • Jung, W.J., Jo, G. H., Kuk, J. H., Kim, K. Y., Park, R. D., 2005. Extraction of chitin from red crab shell waste by cofermentation with Lactobacillus paracasei subsp. tolerans KCTC-3074 and Serratia marcescens FS-3. Applied Microbiology and Biotechnology (71) pp. 234-237.

  • Jung, W.J., Jo, G. H., Kuk, J. H., Kim, Y. J., Oh, K. T., Park, R. D., 2007. Production of chitin from red crab shell waste by successive fermentation with Lactobacillusparacasei KCTC-3074 and Serratia marcescens FS-3. Carbohydrate Polymers (68) pp. 746-750.

  • Oh, K. T., Kim, Y. J., Nguyen, V. N., Jung, W.J., Park, R.D. 2007. Demineralization of crab shell waste by Pseudomonas aeruginosa F722. Process Biochemistry. (42) pp. 1069–1074.

  • Jo, G.H., Park, R.D., Jung, W.J., 2011. Enzymatic production of chitin from crustacean shell waste. Chitin, Chitosan, Oligosaccharides and their derivatives. Boca Raton: CRC Press.

  • Rao, M. S., Muñoz, J., Stevens, W. F., 2000. Critical factors in chitin production by fermentation of shrimp biowaste. Applied Microbiology and Biotechnology (54) pp. 808-813.


Thank you very much for your attention Treatment of Raw Crustacean Waste to Produce Chitinous Materials.


ad