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垂直板聚丙烯酰胺凝胶连续电泳分离乳酸脱氢酶同工酶 (活性染色鉴定法) PowerPoint PPT Presentation


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垂直板聚丙烯酰胺凝胶连续电泳分离乳酸脱氢酶同工酶 (活性染色鉴定法). 实验原理. 关于垂直板聚丙烯酰胺凝胶电泳 关于连续系统和不连续系统 关于乳酸脱氢酶同工酶及酶活性染色. 垂直板聚丙烯酰胺凝胶电泳. 电泳 带电颗粒在电场的作用下,向着与其电性相反的电极方向移动,这种现象称之为电泳。

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垂直板聚丙烯酰胺凝胶连续电泳分离乳酸脱氢酶同工酶 (活性染色鉴定法)

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  • (Acr)N,N-(Bis)(AP)N,N,N,N-(TEMED)


Ap tmted

AP-TMTED

  • TEMEDAP

    S2O822SO4

  • Acr

  • Bis


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APTEMED3060min

pH TEMEDAP

AcrBisAP


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  • TC

    a(g)b(g)m(mL)

  • aba:b10a:b1005%a:b303%


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TC7.5%


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pI


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pHpH


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pH


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1959Markert(LDH)5LDH1LDH2LDH3LDH4LDH5LDHLDHHM

LDHLDHLDH


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LDHLDH(PMS)(NBT)NBTNBTLDH


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1 2 3 4

LDH5

LDH4

LDH3

LDH2

LDH1


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PharmaciaSE250

  • 2


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5(10 000 r /min15 min)40 %4


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180o


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(T=7.5%C=2.6%) AP0.3 cm30~60 min


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125mL250mL


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8L

5L

3L

10L


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80 V

120 V

0.5 cm

0.5h~1h


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3715~30 min3~5 min


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  • 30mL

  • 1000 mL50 mL


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