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Sequenziamento: metodo di Sanger con ddNTP

Sequenziamento: metodo di Sanger con ddNTP. Sequenziamento con dideossonucleotidi. Sequenziamento automatizzato del DNA. Figure 1: Illumina Flow Cell. Figure 2: Prepare Genomic DNA Sample. DNA. Adapters. Figure 3: Attach DNA to Surface. Figure 4: Bridge Amplification. Adapters. DNA.

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Sequenziamento: metodo di Sanger con ddNTP

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  1. Sequenziamento: metodo di Sanger con ddNTP

  2. Sequenziamento con dideossonucleotidi

  3. Sequenziamento automatizzato del DNA

  4. Figure 1: Illumina Flow Cell Figure 2: Prepare Genomic DNA Sample DNA Adapters Figure 3: Attach DNA to Surface Figure 4: Bridge Amplification Adapters DNA Dense lawn of primers

  5. Figure 5: Fragments Become Double Stranded Figure 6: Denature the Double-Standed Molecules Attached terminus Attached Attached terminus Free terminus Attached Figure 7: Complete Amplification Figure 8: Determine First Base Clusters Laser

  6. Figure 9: Image First Base Figure 10: Determine Second Base Figure 11: Image Second Chemistry Cycle Figure 12: Sequencing Over Multiple Chemistry Cycles Figure 13: Align Data Laser GCTGA...

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