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  1. Supplemental material 1. Spraying with ethephon produces an ethylene physiological response. One-month-old seedlings were sprayed with different concentrations of ethephon, without (A) or with (B) a previous application of different concentrations of AgNO3. After each application, the Magenta boxes were hermetically closed and the leaf abscission was evaluated after an 8-day period.

  2. PR1 1 60 Capsicum chinenseTGLFNISLLL .VSLMILAIF HSCDAQNSPQ DYLEVHNNAR AQVGVGPMTW DAGLASRAQN SolanumtuberosumMGLFNISLLL .TCLMVLAIF HSCDAQNSPQ DYLAVHNDAR AQVGVGPMSW DAGLASRAQN SolanumphurejaMGLFNISLLL .TCLMILAIF HSCDAQNSPQ DYLAVHNDAR AQVGVGPMSW DAGLASRAQN Capsicum annuum MGLFNIPLLL .VCLIVLAIF HSCHAQNSPQ DYLAVHNNAR AQVGVGPMSW DAGLASRAQN SolanumlycopersicumMGLFNMSLLLMTCLMVLAIF HSCDAQNSPQ DYLEVHNDAR AQVGVGPMSW DADLESRAQS 61 120 Capsicum chinense YANSRTGDCN LIHSGPGENL AKGGGDFTGR AAVELWVFEK PNYNHGTNQC ASGKVCGHYT SolanumtuberosumYANSRTGDCN LIHSGAGENL AKGTGDFTGR AAVQLWVGEK PNYNYGTNQC ASGQVCGHYT SolanumphurejaYANSRTGDCN LIHSGAGENL AKGSGDFTGRAAVQLWVGEKPNYNYGTNQC ASGQVCGHYT Capsicum annuum YANSRTGDCN LIHSGAGENL AKGGGDYTGRRAVELWVSEK PNYNHATNQCAGGE.CRHYT SolanumlycopersicumYANSRAGDCN LIHSGSGENL AKGGGDFTGR AAVELWVSEK PNYNYDTNECVSGKMCGHYT 121 162 Capsicum chinense QVVWRNSVRL GCGRARCNNG WWFISCNYDP VGNWAGQSPL LK SolanumtuberosumQVVWRNSVRL GCGRARCNNG WWFISCNYDP VGNWVGQRPY SolanumphurejaQVVWRNSVRL GCGRARCNNG WWFISCNYDP VGNYVGQRPY Capsicum annuum QVVWRNSVRL GCGRARCNNG WWFISCNYDP VGNWVGQRPY SolanumlycopersicumQVVWRDSVRL GCGRALCNDG WWFISCNYDP VGNWVGQRPY Supplemental material 2. The deduced amino acid sequence of Habanero pepper PR1cDNA was aligned to their corresponding orthologues in Solanumtuberosum(GenBank accession code AY050221), Solanumphureja(GenBank accession code AJ493450), Capsicum annuum (GenBank accession code AY829644), and Solanumlycopersicum(GenBank accession code AJ001627).

  3. PR10 1 60 Capsicum chinenseMGAYTFTNES TVPVAPSRLF KALVIDFNHI VCKLMP.DVE SIENIEGDGG AGTIKKVTFV Capsicum annuumMGAYTFTDKS TASVAPSRLF KALVIDFNNLVSKLAP.DVKSIENVEGDGG AGTIKKMTFV Capsicum baccatumMGAYTFTDKS TASVAPSRLF KALVIDFNNI VSKLAP.DVKSIENVEGDGG AGTIKKMTFV SolanumvirginianumMGVNTYTHESTTSISPSRLF KALVLHFDNLVPQVVPHEVKNIETIEGDGG AGSIKQMNFV SolanumlycopersicumMGVTTYTHEDTSTVSPNRLF KALVIDGDNL IPKLMP.NVKNVET.EGDG. ..SIKKINFV 61 120 Capsicum chinense EGGPMKYLRH KIHVIDEENL VTKYSLIEGD ALANKADSVD YDVKFEASAD GGCVGTTVTV Capsicum annuum EGGPIKYMKH KIHVIDEKNL VTKYSLIESDVTENKAESVD YDGKFEASAD GGSVCTTVTV Capsicum baccatumEGGPIKYMKH KIHVIDEKNL VTKYSLIESDVLEGKAESVD YDGKFEASAD GGCVCTTVTV SolanumvirginianumEGGPIKYLKH KIHVIDDKNLETKYSLIEGDVLGDKLESINYDIKFEASGD GGCVCKTTTE SolanumlycopersicumEGGPIKYLKH KIHAIDDKNL VTKYSLIEGDVLGDKLESITHDVKFEPAGNGGCVCKTKTE 121 160 Capsicum chinense YNTIGDYVVT EEEHNLHKAR FDAIVTAVVT YLLTNPSAYA Capsicum annuum YNTKGDYVVT EEEHNVHKEKANDLLKAIEAYLLANPSVYV Capsicum baccatumYNTKGDYVVT EEEHNVHKDKANDLLKAIEAYLLANPSVCV SolanumvirginianumYHTKGDHVIT EEEDNVGKDKAINLFKAVEAHLLANPSVYA SolanumlycopersicumYHTKGDYVLKDEEHNEGKKHAMELFKAVEDYLLANPSIYA Supplemental material 3. The deduced amino acid sequence of Habanero pepper PR10cDNA was aligned to their corresponding orthologues in Capsicum annuum (GenBank accession code AF244121), Capsicum baccatum(GenBank accession code DQ351934), Solanumvirginianum(GenBank accession code AY660753), and Solanumlycopersicum(GenBank accession code AB211525).

  4. Esterase 1 60 Capsicum chinenseMDGLYWIKST QDEWVRKYSD LSNVYLFGSS CGGNIAYHAG LRVAAGAYKE LEPVKIKGLI Capsicum annuumMDGLYWIKST QDEWVRKYSD LSNVYLFGSS CGGNIAYHAG LRVAAGAYKE LEPVKIKGLI ActinidiaargutaVDALHWIRTS DDEWLRDFAD LSNCFLMGSS AGGNIAYHAG LRAAAAV.DD LAPLKIQGMV Medicago truncatulaMEALSLIRSS QDEWLTKYVD YSKCYLMGNS AGATIAYHAG LRVLEKV.ND FEPLKIQGLI 61 120 Capsicum chinenseLHQPYFSGKN RTESEEKLKD DQLLPLHAID KMFDLSLPKG TLDHDHEYSN PFLNGG.SKH Capsicum annuumLHQPYFSGKN RTESEEKLKD DQLLPLHAID KMFDLSLPKG TLDHDHEYSN PFLNGG.SKH ActinidiaargutaLHQPYFGGSD RTPSEMRSVD DPLLPLFVNHLMWELSLPIG .ADRDHEYCN LTVSSE.SES Medicago truncatulaLRQPFFGGTN RTESELRLEN DPNFPLCVSD LCWDLALPIG .VDRNHEYCN PTVGNDVDEK 121 180 Capsicum chinenseLDDVIAQGWK ILVTGVSGDP LVDNARNFAN FMEEKGIKTF KLFGD.GYHA IEGFEPSKAA Capsicum annuumLDDVIAQGWK ILVTGVSGDP LVDNARNFAN FMEEKGIKTF KLFGD.GYHA IEGFEPSKAA ActinidiaargutaIETFKLLGWK VIVTGCDGDP LIDRQMELVKVLEKKGVRTIALFDEGGFHGVEFRDPTRMK Medicago truncatulaLDKIKDQGWR VLVSINGGDLLADRAKELVQ LMDEKGVEVVKDFQEEGFHGVEFFEPSKAK 181 195 Capsicum chinenseALIGAPKDFICATTN Capsicum annuumALIGATKDFICATTN ActinidiaargutaAFLEFLIPCL N.... Medicago truncatulaKFIKLVKGFISTFDA Supplemental material 4. The deduced amino acid sequence of Habanero pepper esterase cDNA was aligned to their corresponding orthologues in Caspicum annuum (GenBank accession code AF072533), Actinidiaarguta(GenBank accession code DQ279915), and Medicagotruncatula(GenBank accession code BT052596).

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