Efficacy of hydrogen peroxide to control saprolegniasis on cool and warmwater fish eggs
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Efficacy of hydrogen peroxide to control Saprolegniasis on cool- and warmwater fish eggs. Hydrogen peroxide INAD 10-023 (Perox-Aid TM ). Egg Efficacy Trial Participants. Mark Drobish – Gavins Point NFH Lynn Lee – UMESC Andy Moore – Rathbun Research Clark Moen – Blue Dog SFH

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Efficacy of hydrogen peroxide to control saprolegniasis on cool and warmwater fish eggs

Efficacy of hydrogen peroxide to control Saprolegniasis on cool- and warmwater fish eggs

Hydrogen peroxide INAD 10-023

(Perox-AidTM)


Egg efficacy trial participants
Egg Efficacy Trial Participants cool- and warmwater fish eggs

  • Mark Drobish – Gavins Point NFH

  • Lynn Lee – UMESC

  • Andy Moore – Rathbun Research

  • Clark Moen – Blue Dog SFH

  • Tom Harder – Max McGraw

  • Dennis Smith – Dundee SFH

  • Jerry Hamilton – Blind Pony SFH


Inad 10 023
INAD #10-023 cool- and warmwater fish eggs

  • Study CAP-00-FUNGUS

    • submitted to CVM 03 March 2000

    • Twenty-nine participating facilities

  • Study CAP-00-PARASITES

    • submitted to CVM 18 April 2000

    • Twenty-one participating facilities

  • Study CAP-00-BACTERIA

    • submitted to CVM 18 April 2000

    • Twenty-three participating facilities


Inad 10 023 participant locations

Western Chemicals cool- and warmwater fish eggs

UMESC

Eka Chemicals, Inc

INAD #10-023 Participant Locations


Protocol methods
Protocol Methods cool- and warmwater fish eggs

Monitor / Record Mortality

Identify Disease

“Control”

Station History

“Prevention”

  • Select therapy and treat

  • concentration

  • duration

  • frequency

  • repetition

Chemical Inventory

Controls?

Monitor / Record Mortality

Adverse Reactions


Hydrogen peroxide therapy fish
Hydrogen peroxide therapy - Fish cool- and warmwater fish eggs

  • Duration: 30 or 60 min

  • Concentration

    • 50, 75, or 100 mg/L for 60 min

    • 100 or 150 mg/L for 30 min

  • Repetition/Interval: once daily on consecutive or alternate days for up to 10 treatments.


Hydrogen peroxide therapy eggs
Hydrogen peroxide therapy - Eggs cool- and warmwater fish eggs

  • Duration: 15 min

  • Concentration: 500 or 750 mg/L

    • minimum incubator concentration is 500 mg/L for 15 min

  • Repetition/Interval: Once daily on consecutive or alternate days through hatch


Egg efficacy trials
Egg Efficacy Trials cool- and warmwater fish eggs

  • Seven egg efficacy trials conducted

    • Blind Pony SFH, Jerry Hamilton, paddlefish

    • Blue Dog SFH, Clark Moen, walleye

    • Dundee SFH, Dennis Smith, smallmouth bass

    • Gavins Point NFH, Mark Drobish, walleye

    • Max McGraw, Tom Harder, walleye

    • Rathbun SFH, Alan Moore, channel catfish

    • UMESC, Lynn Lee, walleye

  • Fungus identified in three trials


Saprolegnia parasitica

Gemmae (red arrow) cool- and warmwater fish eggs

Zoospores in zoosporangium

(blue arrow)

Saprolegnia parasitica

Photo credit: Steve Carpenter, Ph.D., Abbey Lane Labs.


Blind pony sfh paddlefish
Blind Pony SFH - paddlefish cool- and warmwater fish eggs

  • Temperature 15 - 16°C

  • Four trials - one or two females per trial

    • 500, 750, or 1000 mg/L

    • % hatch ranged from 14 - 56% (of fertile eggs)

    • 2 - 4 consecutive treatments; fungus in all treated jars

    • noted cessation of egg development after hydrogen peroxide treatment

    • withholding treatments until 2 d post fertilization resulted in basically no hatch

    • compared rolling w/o treatment to hydrogen peroxide treatment w/o rolling

      • rolled eggs - 60% hatch treated eggs 24% hatch


Blind pony paddlefish saprolegnia ferax
Blind Pony - paddlefish cool- and warmwater fish eggsSaprolegnia ferax

Oogonium with oospores showing cell wall pitting.

Oogonia and gemmae from sterile house fly culture.

Photo credit: Steve Carpenter, Ph.D., Abbey Lane Labs.


Max mcgraw walleye
Max McGraw - walleye cool- and warmwater fish eggs

~ 1 million eggs total

x = 72%

x = 68%


Blue dog sfh walleye
Blue Dog SFH - walleye cool- and warmwater fish eggs

~1.5 million eggs total


Gavins point nfh walleye
Gavins Point NFH - walleye cool- and warmwater fish eggs

~3.1 million eggs total


Gavins point nfh walleye1

Control cool- and warmwater fish eggs

500 mg/L

750 mg/L

Gavins Point NFH - walleye

Photo credit: Mark Drobish, Gavins Point NFH


Umesc walleye
UMESC - walleye cool- and warmwater fish eggs

~2.7 million eggs total


Umesc walleye1
UMESC - Walleye cool- and warmwater fish eggs


Dundee sfh smallmouth bass eggs
Dundee SFH - cool- and warmwater fish eggssmallmouth bass eggs

~ 2500 eggs per jar

~30,000 eggs total


Dundee sfh smallmouth bass eggs1
Dundee SFH - cool- and warmwater fish eggssmallmouth bass eggs

Number of fungused eggs removed during incubation.


Dundee sfh smallmouth bass fry
Dundee SFH - cool- and warmwater fish eggssmallmouth bass fry

~ 2500 fry per tank

~30,000 fry total


Rathbun sfh channel catfish eggs
Rathbun SFH - cool- and warmwater fish eggschannel catfish eggs


Rathbun sfh channel catfish control eggs
Rathbun SFH - cool- and warmwater fish eggschannel catfish control eggs

Photo credit: Andy Moore, Rathbun SFH


Rathbun sfh channel catfish treated eggs
Rathbun SFH - cool- and warmwater fish eggschannel catfish treated eggs

Photo credit: Andy Moore, Rathbun SFH


Conclusions
Conclusions cool- and warmwater fish eggs

  • A hydrogen peroxide treatment regimen of 500 mg/L administered for 15 min on consecutive days from incubation to hatch is effective in controlling fungus on walleye and channel catfish eggs.

  • Hydrogen peroxide virtually eliminated fungal growth in both walleye and channel catfish eggs and increased the probability of walleye hatch in two of three controlled trials.


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