efficacy of hydrogen peroxide to control saprolegniasis on cool and warmwater fish eggs
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Efficacy of hydrogen peroxide to control Saprolegniasis on cool- and warmwater fish eggs. Hydrogen peroxide INAD 10-023 (Perox-Aid TM ). Egg Efficacy Trial Participants. Mark Drobish – Gavins Point NFH Lynn Lee – UMESC Andy Moore – Rathbun Research Clark Moen – Blue Dog SFH

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efficacy of hydrogen peroxide to control saprolegniasis on cool and warmwater fish eggs

Efficacy of hydrogen peroxide to control Saprolegniasis on cool- and warmwater fish eggs

Hydrogen peroxide INAD 10-023

(Perox-AidTM)

egg efficacy trial participants
Egg Efficacy Trial Participants
  • Mark Drobish – Gavins Point NFH
  • Lynn Lee – UMESC
  • Andy Moore – Rathbun Research
  • Clark Moen – Blue Dog SFH
  • Tom Harder – Max McGraw
  • Dennis Smith – Dundee SFH
  • Jerry Hamilton – Blind Pony SFH
inad 10 023
INAD #10-023
  • Study CAP-00-FUNGUS
    • submitted to CVM 03 March 2000
    • Twenty-nine participating facilities
  • Study CAP-00-PARASITES
    • submitted to CVM 18 April 2000
    • Twenty-one participating facilities
  • Study CAP-00-BACTERIA
    • submitted to CVM 18 April 2000
    • Twenty-three participating facilities
protocol methods
Protocol Methods

Monitor / Record Mortality

Identify Disease

“Control”

Station History

“Prevention”

  • Select therapy and treat
  • concentration
  • duration
  • frequency
  • repetition

Chemical Inventory

Controls?

Monitor / Record Mortality

Adverse Reactions

hydrogen peroxide therapy fish
Hydrogen peroxide therapy - Fish
  • Duration: 30 or 60 min
  • Concentration
    • 50, 75, or 100 mg/L for 60 min
    • 100 or 150 mg/L for 30 min
  • Repetition/Interval: once daily on consecutive or alternate days for up to 10 treatments.
hydrogen peroxide therapy eggs
Hydrogen peroxide therapy - Eggs
  • Duration: 15 min
  • Concentration: 500 or 750 mg/L
    • minimum incubator concentration is 500 mg/L for 15 min
  • Repetition/Interval: Once daily on consecutive or alternate days through hatch
egg efficacy trials
Egg Efficacy Trials
  • Seven egg efficacy trials conducted
    • Blind Pony SFH, Jerry Hamilton, paddlefish
    • Blue Dog SFH, Clark Moen, walleye
    • Dundee SFH, Dennis Smith, smallmouth bass
    • Gavins Point NFH, Mark Drobish, walleye
    • Max McGraw, Tom Harder, walleye
    • Rathbun SFH, Alan Moore, channel catfish
    • UMESC, Lynn Lee, walleye
  • Fungus identified in three trials
saprolegnia parasitica

Gemmae (red arrow)

Zoospores in zoosporangium

(blue arrow)

Saprolegnia parasitica

Photo credit: Steve Carpenter, Ph.D., Abbey Lane Labs.

blind pony sfh paddlefish
Blind Pony SFH - paddlefish
  • Temperature 15 - 16°C
  • Four trials - one or two females per trial
    • 500, 750, or 1000 mg/L
    • % hatch ranged from 14 - 56% (of fertile eggs)
    • 2 - 4 consecutive treatments; fungus in all treated jars
    • noted cessation of egg development after hydrogen peroxide treatment
    • withholding treatments until 2 d post fertilization resulted in basically no hatch
    • compared rolling w/o treatment to hydrogen peroxide treatment w/o rolling
      • rolled eggs - 60% hatch treated eggs 24% hatch
blind pony paddlefish saprolegnia ferax
Blind Pony - paddlefishSaprolegnia ferax

Oogonium with oospores showing cell wall pitting.

Oogonia and gemmae from sterile house fly culture.

Photo credit: Steve Carpenter, Ph.D., Abbey Lane Labs.

max mcgraw walleye
Max McGraw - walleye

~ 1 million eggs total

x = 72%

x = 68%

blue dog sfh walleye
Blue Dog SFH - walleye

~1.5 million eggs total

gavins point nfh walleye
Gavins Point NFH - walleye

~3.1 million eggs total

gavins point nfh walleye1

Control

500 mg/L

750 mg/L

Gavins Point NFH - walleye

Photo credit: Mark Drobish, Gavins Point NFH

umesc walleye
UMESC - walleye

~2.7 million eggs total

dundee sfh smallmouth bass eggs
Dundee SFH - smallmouth bass eggs

~ 2500 eggs per jar

~30,000 eggs total

dundee sfh smallmouth bass eggs1
Dundee SFH - smallmouth bass eggs

Number of fungused eggs removed during incubation.

dundee sfh smallmouth bass fry
Dundee SFH - smallmouth bass fry

~ 2500 fry per tank

~30,000 fry total

rathbun sfh channel catfish control eggs
Rathbun SFH - channel catfish control eggs

Photo credit: Andy Moore, Rathbun SFH

rathbun sfh channel catfish treated eggs
Rathbun SFH - channel catfish treated eggs

Photo credit: Andy Moore, Rathbun SFH

conclusions
Conclusions
  • A hydrogen peroxide treatment regimen of 500 mg/L administered for 15 min on consecutive days from incubation to hatch is effective in controlling fungus on walleye and channel catfish eggs.
  • Hydrogen peroxide virtually eliminated fungal growth in both walleye and channel catfish eggs and increased the probability of walleye hatch in two of three controlled trials.
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