Oxidative phosphorylation Biochemistry, 4 th edition, RH Garrett & CM Grisham,

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Oxidative phosphorylation Biochemistry, 4 th edition, RH Garrett & CM Grisham,

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Oxidative phosphorylation Biochemistry, 4 th edition, RH Garrett & CM Grisham,

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Oxidative phosphorylation

Biochemistry, 4th edition, RH Garrett & CM Grisham,

Brooks/Cole (Cengage); Boston, MA: 2010

pp 592-629

Instructor: Kirill Popov

The mitochondrion

Electron transport

Oxidative phosphorylation

Heat, oxidative stress, etc.

*Numbers of subunits in the bacterial equivalents in parentheses.

#Cytochromec is not part of an enzyme complex; it moves between Complexes III and IV as a freely soluble protein.

The P/O ratio is an index of the efficiency of coupling

P/O ratio: number of molecules of Pi incorporated (=ATP synthesized) per

atom of oxygen consumed (or pair of electrons being carried through the chain).

Measurements: oxygen consumption during complete phosphorylation

of a fixed amount of ADP after addition of either an NAD+-linked substrate or FAD-linked substrate.

P/O 2.5 for NADH

P/O 1.5 for FADH2

*The number depends on which shuttle system transfers reducing equivalents into the mitochondrion.