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Ex. 16 Oxygen Requirements. Oxygen Requirements. 1. Aerobes 2. Anaerobes 3. Facultative 4. Microaerophilic. Agar Stabs & Agar Shakes. 1. Liquify 6 NA Deeps in boiling water 2. Boil for 10 minutes to drive out dissolved oxygen 3. Allow 3 NA Deeps to cool and solidify Agar Stabs

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oxygen requirements
Oxygen Requirements
  • 1. Aerobes
  • 2. Anaerobes
  • 3. Facultative
  • 4. Microaerophilic
agar stabs agar shakes
Agar Stabs & Agar Shakes
  • 1. Liquify 6 NA Deeps in boiling water
  • 2. Boil for 10 minutes to drive out dissolved oxygen
  • 3. Allow 3 NA Deeps to cool and solidify
    • Agar Stabs
  • 4. Temper 3 NA Deeps in water bath at 55 degrees C
    • Agar Shakes
agar stabs
Agar Stabs
  • 1. Single stab with Inoculating needle – straight down, straight back up
  • 1. Micrococcus luteus
  • 2. Staphylococcus epidermidis
  • 3. Clostridium sporogenes
  • Clostridium rubrum
  • Clostridium butrycum
agar shakes
Agar Shakes
  • 1. Inoculate with Inoculating Loop - “shake” test tube so bacteria are equally distributed
  • 1. Micrococcus luteus
  • 2. Staphylococcus epidermidis
  • 3. Clostridium sporogenes
  • Clostridium rubrum
  • Clostridium butrycum
agar stab
Agar Stab
  • Aerobic
agar stab1
Agar Stab
  • Anaerobic
agar stab2
Agar Stab
  • Facultative
agar stab3
Agar Stab
  • Microaerophilic
fluid thioglycollate broths ftb
Fluid Thioglycollate Broths (FTB)
  • Sodium thioglycollate
    • Binds to and removes free oxygen
  • Resazurin
    • Anaerobic indicator
      • O2 - PINK
      • No O2 - Clear
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