Ex 16 oxygen requirements
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Ex. 16 Oxygen Requirements. Oxygen Requirements. 1. Aerobes 2. Anaerobes 3. Facultative 4. Microaerophilic. Agar Stabs & Agar Shakes. 1. Liquify 6 NA Deeps in boiling water 2. Boil for 10 minutes to drive out dissolved oxygen 3. Allow 3 NA Deeps to cool and solidify Agar Stabs

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Oxygen requirements
Oxygen Requirements

  • 1. Aerobes

  • 2. Anaerobes

  • 3. Facultative

  • 4. Microaerophilic


Agar stabs agar shakes
Agar Stabs & Agar Shakes

  • 1. Liquify 6 NA Deeps in boiling water

  • 2. Boil for 10 minutes to drive out dissolved oxygen

  • 3. Allow 3 NA Deeps to cool and solidify

    • Agar Stabs

  • 4. Temper 3 NA Deeps in water bath at 55 degrees C

    • Agar Shakes


Agar stabs
Agar Stabs

  • 1. Single stab with Inoculating needle – straight down, straight back up

  • 1. Micrococcus luteus

  • 2. Staphylococcus epidermidis

  • 3. Clostridium sporogenes

  • Clostridium rubrum

  • Clostridium butrycum


Agar shakes
Agar Shakes

  • 1. Inoculate with Inoculating Loop - “shake” test tube so bacteria are equally distributed

  • 1. Micrococcus luteus

  • 2. Staphylococcus epidermidis

  • 3. Clostridium sporogenes

  • Clostridium rubrum

  • Clostridium butrycum


Agar stab
Agar Stab

  • Aerobic


Agar stab1
Agar Stab

  • Anaerobic


Agar stab2
Agar Stab

  • Facultative


Agar stab3
Agar Stab

  • Microaerophilic


Fluid thioglycollate broths ftb
Fluid Thioglycollate Broths (FTB)

  • Sodium thioglycollate

    • Binds to and removes free oxygen

  • Resazurin

    • Anaerobic indicator

      • O2 - PINK

      • No O2 - Clear


Oxygen reqirements
Oxygen Reqirements


Aerobic microaerophilic anaerobic
Aerobic Microaerophilic Anaerobic


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