Restriction mapping
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Restriction Mapping. Restriction Mapping: Double Digests. Factor I – Single Cut. Factor 2 – Single Cut. Restriction Mapping: Double Digests. Restriction Mapping: Double Digests. Restriction Mapping: Double Digests. Uses of Restriction Mapping. Fingerprinting

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Restriction Mapping

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Restriction Mapping


Restriction Mapping:Double Digests

Factor I – Single Cut

Factor 2 – Single Cut


Restriction Mapping:Double Digests


Restriction Mapping:Double Digests


Restriction Mapping:Double Digests


Uses of Restriction Mapping

  • Fingerprinting

  • Restriction Fragment Length Polymorphism Mapping


Cloning


pUC19


pUC19 Multiple Cloning Site


AGCT

PLASMID

TCGA

AGCT

KAN

TCGA

Ligation


AGCT

PLASMID

TCGA

AGCT

KAN

TCGA

Ligation

DNA ligase


AGCT

PLASMID

TCGA

AGCT

KAN

TCGA

Ligation


AGCT

PLASMID

TCGA

AGCT

KAN

TCGA

Ligation


Ligation


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