Restriction mapping
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Restriction Mapping. Restriction Mapping: Double Digests. Factor I – Single Cut. Factor 2 – Single Cut. Restriction Mapping: Double Digests. Restriction Mapping: Double Digests. Restriction Mapping: Double Digests. Uses of Restriction Mapping. Fingerprinting

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Restriction Mapping

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Restriction mapping

Restriction Mapping


Restriction mapping double digests

Restriction Mapping:Double Digests

Factor I – Single Cut

Factor 2 – Single Cut


Restriction mapping double digests1

Restriction Mapping:Double Digests


Restriction mapping double digests2

Restriction Mapping:Double Digests


Restriction mapping double digests3

Restriction Mapping:Double Digests


Uses of restriction mapping

Uses of Restriction Mapping

  • Fingerprinting

  • Restriction Fragment Length Polymorphism Mapping


Cloning

Cloning


Puc19

pUC19


Puc19 multiple cloning site

pUC19 Multiple Cloning Site


Ligation

AGCT

PLASMID

TCGA

AGCT

KAN

TCGA

Ligation


Ligation1

AGCT

PLASMID

TCGA

AGCT

KAN

TCGA

Ligation

DNA ligase


Ligation2

AGCT

PLASMID

TCGA

AGCT

KAN

TCGA

Ligation


Ligation3

AGCT

PLASMID

TCGA

AGCT

KAN

TCGA

Ligation


Ligation4

Ligation


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