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Restriction Mapping. Restriction Mapping: Double Digests. Factor I – Single Cut. Factor 2 – Single Cut. Restriction Mapping: Double Digests. Restriction Mapping: Double Digests. Restriction Mapping: Double Digests. Uses of Restriction Mapping. Fingerprinting

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Presentation Transcript
restriction mapping double digests
Restriction Mapping:Double Digests

Factor I – Single Cut

Factor 2 – Single Cut

uses of restriction mapping
Uses of Restriction Mapping
  • Fingerprinting
  • Restriction Fragment Length Polymorphism Mapping
ligation

AGCT

PLASMID

TCGA

AGCT

KAN

TCGA

Ligation
ligation1

AGCT

PLASMID

TCGA

AGCT

KAN

TCGA

Ligation

DNA ligase

ligation2

AGCT

PLASMID

TCGA

AGCT

KAN

TCGA

Ligation
ligation3

AGCT

PLASMID

TCGA

AGCT

KAN

TCGA

Ligation
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