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Team Chem. Biological Water Filtration. Purification Process. Add cells to possibly contaminated water Cells take in arsenic and store it Cells provide an output (color) signaling the water is clean or needs further processing Separate of cells from water

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Team chem

Team Chem

Biological Water Filtration


Purification process

Purification Process

  • Add cells to possibly contaminated water

  • Cells take in arsenic and store it

  • Cells provide an output (color) signaling the water is clean or needs further processing

  • Separate of cells from water

  • Possibility for further processing


Devices

Devices

  • 2 Arsenic Sensors

    • Sensitive: activated by any arsenic

    • Non-sensitive: activated by maximum tolerable arsenic level

  • 2 Color Generators

  • Arsenic Transport and Storage Device


Arsenic transport and storage

Arsenic Transport and Storage

Arsenic Measurement

Remove the gene that encodes this transport protein

Tripathi, R.D., Srivastava, S., Mishra, S., Singh, N., Tuli, R., Gupta, D.K. and Maathuis, F. Arsenic Hazards: Strategies for Tolerance and Remediation by Plants. Trends in Biotechnology. 2007, 25.4, 158-165.


Device level diagram

Where is Concentration Read?

Sensitive Arsenic Sensor

Arsenic Transport and Storage Device

(constitutively active)

Non-Sensitive Arsenic Sensor

Device Level Diagram

Color Generator 1

(constitutively active)

Outside Vacuole

Inside Vacuole or with a Timer

Color Generator 2


Simulation pure water

Sensitive Arsenic Sensor

Arsenic Transport and Storage Device

(constitutively active)

Non-Sensitive Arsenic Sensor

Simulation: Pure Water

Green Color Generator

(constitutively active)

Red Color Generator

Result: Cells Stay Green → Separation


Simulation maximum arsenic

Sensitive Arsenic Sensor

As

As

Arsenic Transport and Storage Device

(constitutively active)

MAXIMUM

ARSENIC

Non-Sensitive Arsenic Sensor

Simulation: Maximum Arsenic

Green Color Generator

(constitutively active)

Red Color Generator

Result: Cells Turn Red → Separate and Process Again


Timing diagram maximum arsenic

1

1

1

1

1

Green Color Generator

0

0

0

0

0

Sensitive [As] Sensor

Transport and Storage

Non- Sensitive [As] Sensor

Red Color Generator

t=0

No Arsenic

Timing Diagram: Maximum Arsenic

Contaminated Water Added

Midlevel [As]

Maximum [As]


Timing diagram midlevel arsenic

1

1

1

1

1

Green Color Generator

0

0

0

0

0

Sensitive [As] Sensor

Transport and Storage

Non- Sensitive [As] Sensor

Red Color Generator

t=0

No Arsenic

Timing Diagram: Midlevel Arsenic

Contaminated Water Added

Midlevel [As]

Maximum [As]


Biological parts background system

Biological PartsBackground System

ARR2

ARR1


Biological parts control system

Biological PartsControl System

P(x)

YCF1

GSH1

P(y)

GCGD:

BBa_K274004

[As]

[As]max

P(z)

RCGD:

BBa_K274100

Y-Repressor

X-Repressor


Cellular chassis

Cellular Chassis

[Source:http://www.botany.hawaii.edu/nlc_biology/1406/lab/r2/slide6.jpg]

[Source: http://www.ppws.vt.edu/scott/weed_id/eldde.htm]


Testing debugging

Testing/Debugging

[Source: http://www.sciencephoto.com/images/]

  • Cell Death

    • Effectiveness of ARR1, ARR1, YCF1, GSH1

  • Need to determine Vacuole limits

  • Testing Color Generators

  • Promoter Design


Global impacts

Global Impacts


Impacts

Impacts

If this process succeeds:

  • Solves one of the most problematic issues of the developing world, saving millions of lives.

    • 70 million people are affected in Bangladesh alone - arsenic in ground water is the cause of 23% of all the deaths there!


Why biological water filtration by team chemistry

Why Biological Water Filtration by Team Chemistry?

  • No energy requirement (operates independent of electricity)

    • Accessibility to effective water filtration technology even in rural parts of the developing world

  • Very low cost of sustaining system

  • The end product is not a waste

    • Possibility of selling to pharmaceutical companies and turning a profit


Open issues

Open Issues

  • Elodea is the current choice of organism

    • Common water weed: robust, packaged for surviving in water

  • The cost of entire project is unknown.

  • Legal issues attached to introducing genetically modified organisms into the environment?

  • Length of effectiveness is unknown

    • (How long until the organism reaches max arsenic uptake capacity?)

  • The process of genetically modifying plants is very slow…


Go no go

Go/ No Go?


Citations

Citations

Saccharomyces Genome Database www.yeastgenome.org

Standard Registry of Biological Parts www.partregistry.org


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