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Biological Safety: Safety for people, animals, plants. BioSafety and BioHazard Training. Office of Engineering Safety Texas Engineering Experiment Station (TEES) & The Dwight Look College of Engineering. “A safe, healthful, and secure environment for scholarship and research.”. Key Concepts.

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Office of Engineering Safety Texas Engineering Experiment Station (TEES) & The Dwight Look College of Engineering

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Biological Safety: Safety for people, animals, plants

BioSafety and BioHazard Training

Office of Engineering Safety Texas Engineering Experiment Station (TEES)& The Dwight Look College of Engineering

“A safe, healthful, and secure environment for scholarship and research.”


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Key Concepts

  • Hazardous Biological Substances (Biohazards)

  • Bloodborne Pathogens

  • Universal Precautions

  • Exposure Control Plan

  • Biosafety in Microbiological and Biomedical Laboratories (BMBL)

  • Biological Risk Assessment

  • BioSafety Level (BSL)Criteria

  • BioSecurity Criteria

  • Select Agents & Toxins

  • BioHazard Containment, Decontamination & Disinfection

  • TAMU Biological Safety Office


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Why Is Biological Safety Important?

  • Use of hazardous biological materials on campus including

    • Biohazardous agents and bloodborne pathogens

  • Bloodborne Pathogens

  • Prevent disease in personnel

  • Prevent release of organisms/agents into environment


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What are BioHazardous Materials?

  • Any biological material capable of causing harm to humans, animals, plants

  • BioHazardous Agents – Pathogens that can replicate & cause disease include:

    • Bacteria (Streptococcus pyogenes)

    • Fungi (Candida, Histoplasma)

    • Viruses (HIV, HBV)

    • Prions (CJD)

    • Parasites (Giardia, Strongyloides)

  • Toxins – Microbial poisons

    • Exotoxins – produced by bacteria

      • Clostridium botulinum - food poisoning, one of most deadly

      • Clostridium tetanii – tetanus

      • Corynebacterium diphtheriae – diptheria

      • Bioterrorism use

    • Endotoxins – released from cell wall when bacteria disintegrates


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Classification System For Biohazardous MaterialsBioSafety Levels (BSL)


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The Standard Code of Practice for BioSafety

  • Biosafety in Microbiological and Biomedical Laboratories (BMBL)

    • U.S. Department of Health & Human Services

    • Public Health Service

    • Centers for Disease Control & Prevention & the National Institutes of Health

    • http://www.cdc.gov/od/ohs/biosfty/bmbl5/bmbl5toc.htm

  • Establishes criteria for:

    • Biological Risk Assessment

    • Principles of BioSafety

    • Laboratory BioSafety Level (BSL) Criteria

    • Laboratory BioSecurity Criteria

    • BioHazard Containment

    • Decontamination & Disinfection

    • Transportation of BioHazards & Infectious Materials

    • Select Agents & Toxins…


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Researcher Responsibilities:Faculty or Principle Investigator (PI)

  • Files a Biological Usage Authorization (BUA)

  • Trains all personnel

  • Establishes emergency procedures

  • Reports incidents with biologicals

  • Posts biohazard warning signs

  • Cooperates with Biological Safety Officer during inspection visits

  • Responsibilities of TAMU PI’s

    • http://researchcompliance.tamu.edu/ibc/ibcpiresp


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BioSafety Officer TAMU Office of Research Compliance

  • Dr. Bruce M. Whitney

    • Institutional Biological Safety Officer

    • Office of Biological Safety

    • Texas A&M University

      • Tel:979-458-0683 or 436-1307

      • Mailstop:1112 TAMU

      • Email:[email protected]

      • Email:[email protected]

      • http://biosafety.tamu.edu/institutional-biosafety-committee/ibcrevapp/ibcapprev


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Institutional BioSafety Committee (IBC)TAMU Office of Research Compliance

  • Mandated by National Institutes of Heath (NIH)

  • Must review and approve all usage of Risk Group 2 or higher agents

  • Must review and approve rDNA usage, formation of transgenic animals, experiments involving human gene therapy

  • Applies to TAMU:

    • Employees & students conducting research involving infectious biohazrds

    • Research subjects, human or animal

  • TAMU IBC: http://researchcompliance.tamu.edu/ibc/welcome

  • http://biosafety.tamu.edu/institutional-biosafety-committee/ibcrevapp/ibcapprev


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Biological Usage Authorization (BUA)

  • Describes the Principle Investigator’s Research Project

  • Required when biohazardous material is stored, used, or transported

  • Good for 3 years

  • Can be amended


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Cannot be brought on campus!

Mycobacterium tuberculosis

Hantavirus

Foreign Animal Pathogens

HIV 1 & 2

Reference material can be kept in inactive state (frozen or lyophilized)

List of all references must be given to IBC

Forbidden Agents


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CDC Select Agents

  • Cannot be brought on campus without prior approval & registration

  • Must be registered with the University BioSafety Officer

  • Required under:

    • U.S. BioTerrorism Prevention Act

    • USA Patriot Act (USAPA)

    • TAMUS Regulations, TEES & TAMU Rules


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The BioSafety Inspection!

  • Personal Protective Equipment

  • Training

  • Signage

  • Adherence to Protocol

  • Technique

  • Physical Facility

  • General Safety

  • Risk Assessment

  • Controls

  • Project Safety Analysis (PSA)


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What You Must Learn to Work Safely with Bloodborne Pathogens

  • Cleaning

  • Decontamination

  • Regulated waste disposal

  • Pre and post exposure procedures

  • Universal Precautions (CDC)

  • Special hazards

    • HIV, HBV research


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What are Bloodborne Pathogens?

  • Microbial agents transmitted by the blood and bodily fluids of infected individuals.

  • Control of Occupational Exposures to Bloodborne Pathogens

  • 29 CFR 1910.1030

  • http://www.osha.gov/SLTC/bloodbornepathogens/index.html


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Exposure Control Plan

  • All laboratory supervisors, faculty & principle investigators are responsible for insuring their employees and students comply with the Exposure Control Plan to reduce the risk of occupational exposures to bloodborne pathogens.

  • All personnel that have the potential to be exposed to bloodborne pathogens MUST be trained to this plan.

  • A copy of the Exposure Control Plan must be readily available in each work area or laboratory where bloodborne pathogens exposures exist.


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Where to Get More Informationwww.osha.gov/SLTC/bloodbornepathogens/index.html

  • Workers in many different occupations are at risk of exposure to bloodborne pathogens, including Hepatitis B, Hepatitis C, and HIV/AIDS.

  • In 1991, OSHA issued the Bloodborne Pathogens Standard to protect workers from this risk.

  • In 2001, in response to the Needlestick Safety and Prevention Act, OSHA revised the Bloodborne Pathogens Standard, 29 CFR 1910.1030.

  • The revised standard clarifies the need for employers to select safer needle devices and to involve employees in identifying and choosing these devices.

  • The updated standard also requires employers to maintain a log of injuries from contaminated sharps.


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Where to Get More Traininglabsafety.tamu.edu/bloodborne.asp

  • Prevention of Exposures to Bloodborne Pathogens (BBP):

    • Exposure to human blood and bodily fluids can result in transmission of several bloodborne illnesses, including HIV and Hepatitis B.

    • This training presentation will aid in increasing awareness of the risk of exposure to bloodborne pathogens and techniques and practices for preventing exposure and abating hazards in Engineering laboratories and facilities.


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Prepared by:

http://engineering.tamu.edu/safety/


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Biological Safety: Safety for people, animals, plants

BioSafety and BioHazard Training

Office of Engineering Safety Texas Engineering Experiment Station (TEES)& The Dwight Look College of Engineering

“A safe, healthful, and secure environment for scholarship and research.”


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