Strawberry dna
Download
1 / 22

Strawberry DNA - PowerPoint PPT Presentation


  • 543 Views
  • Updated On :

Strawberry DNA. Plant Genomics. Genomics – The study of DNA. Plant chromosomal DNA Chromosome number Plant genes Plant reproduction Plant gene expression – the regulation of genes. Why Plant Genomics.

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about 'Strawberry DNA' - lotus


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
Strawberry dna l.jpg

Strawberry DNA

Plant Genomics


Genomics the study of dna l.jpg
Genomics – The study of DNA

  • Plant chromosomal DNA

  • Chromosome number

  • Plant genes

  • Plant reproduction

  • Plant gene expression – the regulation of genes


Why plant genomics l.jpg
Why Plant Genomics

  • Agricultural applications – Production of healthier crops- more nutritious( Genetic engineering of crop plants

  • Production of crops with disease resistance

  • Pharmacology - What novel genes do plants have to apply to human pharmacological research? Many contain anti- cancer compounds

  • Bioremediation – Plants removing pollutants from the environment


Genomic dna l.jpg
Genomic DNA

  • 30,000 genes

  • Satellite DNA – around the centromeres

  • Telomeric DNA – repetitive copies of

    TTAGGG

  • VNTR – variable number of tandem repeats- variable per species

  • Retrotransposons – remains of ancient retroviruses – are capable of replicating and making enzymes capable of jumping our of one position and finding a new position in DNA


Genomic dna5 l.jpg
Genomic DNA

  • Sines- short interspersed elements – 500 bp but are not translated

  • Lines – long interspersed elements – up to 7000 bases some are transcribed and translated

  • Transposons- move around in the DNA

  • ALUs – a special type of DNA – 300 bp – accounts for 11 % of the human genome


Contradictions to the central dogma l.jpg
Contradictions to the Central Dogma

  • Retroviruses – Other RNA viruses

  • Transposons

  • Other elements in DNA - Alus

  • Prions ( proteins – Mad Cow)

  • Genes for t- RNA

  • Genes for Ribosomal RNAs

  • Spliceosomes and catalytic RNA’s

  • Enhancers and repressors

  • Promoters


Materials l.jpg
Materials

  • Clean blue tube

  • Sharpie marker

  • Eppendorf holder( Microfuge tubes)

  • Loading dye ( green and yellow tube)

  • Tracking dye – white tube TD

  • Marker – white tube – M

  • Strawberry DNA- sample from extraction


Extraction of dna i l.jpg
Extraction of DNA I

  • Homogenize strawberries

  • Filter the stawberry homogenate

  • Place extract in Corning Tube

  • Add lysis mixture – detergent containing lauryl sulfate and salt, NaCl

  • Add papain mixture to denature proteins( DNAses)

  • Mix by rocking and rolling


Extraction of dna ii l.jpg
Extraction of DNA II

  • Heat at 55oC. This speeds up degradation of proteins( 2 min)

  • Place in ice until cold

  • Add ice cold ethanol. Drip slowly down the side of the Corning tube making sure that the alcohol forms a layer on top of the juice.

  • This should form an interface between the two layers.


Dna precipitate iii l.jpg
DNA Precipitate III

  • The DNA should precipitate and form a mass of slender,sticky strands

  • Remove the DNA from the Corning tube, being careful not to disturb the interface

  • The DNA should be placed in a microcentrifuge tube,

  • Centrifuge for 2 minutes


Pellet and supernatant l.jpg
Pellet and Supernatant

  • Spin the tube with the DNA

  • It forms a pellet on the side of the microcentrifuge tube

  • Pour off the supernatant which is alcohol

  • The DNA needs to be resolubilized in Tris buffer

  • Mix the DNA from the pellet with Tris


Tris and dna l.jpg
Tris and DNA

  • The DNA must be in solution for electrophoresis.

  • Mix before using in the gel

  • Remove 25 ul of DNA and place in a microcentrifuge tube.

  • Add 3 ul of loading dye

  • Vortex to mix


Genomic dna gel lanes label on your index card l.jpg
Genomic DNA – Gel LanesLabel on your index card

  • 1-Tracking dye

  • 2- Marker – Lambda phage - HindII

  • 3 -8- Strawberry DNA samples( genomic DNA)

Name on Ziplock Snack Bag for Gel


Loading gels l.jpg
Loading gels

  • Run DNA from black to red

  • From the cathode to the anode

  • Remember DNA has a negative charge


Reminders about loading gel l.jpg
Reminders about loading gel

  • Aspirate to first stop point

  • Deliver by dispensing into the well to the second stop point

  • Fill the well – being careful not to overfill

  • Run the gel on a voltage of 80 volts


Staining l.jpg
Staining

  • Stain with Methylene blue. Fast Blast Stain. Gel should stain for at least one hour

  • Destain with Distilled water

  • Place gel in ziplock bag with water.

  • When gel is sufficeintly destained - the stained gel can be placed in a ziplock for storage in the refrigerator


Gel observation l.jpg
Gel Observation

  • Observe gel on light box

  • Measure the distance that each band in the marker( standard) migrates

  • Measure any bands oar streaks of DNA you observe.

  • Use the DNA graphing program to make a semi-log graph to estimate the sizes of your DNA



ad