陳思妤
This presentation is the property of its rightful owner.
Sponsored Links
1 / 2

Biomedical Optical Imaging Laboratory PowerPoint PPT Presentation


  • 50 Views
  • Uploaded on
  • Presentation posted in: General

陳思妤 Szu-Yu Chen. Superresolution Microscopy: 3D STED Microscopy. STED: S timulated E mission D epletion. Excitation. Stimulated depletion. Emission. x. 4 Pi microscopy Stimulated depletion in z direction Improve axial resolution Goal:

Download Presentation

Biomedical Optical Imaging Laboratory

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -

Presentation Transcript


Biomedical optical imaging laboratory

陳思妤Szu-Yu Chen

Superresolution Microscopy: 3D STED Microscopy

STED: Stimulated Emission Depletion

Excitation

Stimulated depletion

Emission

x

  • 4 Pi microscopy

  • Stimulated depletion in z direction

  • Improve axial resolution

  • Goal:

  • Using one objective instead of two opposing objectives

  • In vivo imaging

x

+

+

=

z

y

2D STED microscopy  improve lateral resolution

Superresolution Microscopy: Laser Scanning SIM

SIM: Structure Illumination Microscopy

  • Laser Scanning SIM:

  • Pixel-by-pixel illumination

  • Easy to be combined with nonlinear microscopy

Image reconstruction

=

+

Sample

Image

2D scanning system

Microscope

  • Conventional SIM:

  • Wide-field illumination

  • Hard to be combined with nonlinear microscopy

  • Moving focal plane in axial:

  • Axial SIM excitation

  • 3D SIM

Biomedical Optical Imaging Laboratory

E-MAIL : [email protected] : 03-4252897TEL : 03-4227151 ext 65276Office : IL-529


Biomedical optical imaging laboratory

陳思妤Szu-Yu Chen

4D Hyperspectral microscopy

Image:

Intensity recorded pixel-by-pixel within certain wavelength range

Spectrum:

Intensity recorded for a single spot at multiple wavelength

Hyperspectral image:

Intensity recorded at multiple wavelength pixel-by-pixel

Image (Y)

Detector

Spectrometer

2D detector array

Wavelength (nm)

Image (X)

+

  • Biomedical information:

  • Morphological (intensity) and molecular (spectral) information

  • Biomedical applications:

  • Molecular imaging

  • Disease diagnosis and treatment

Conventional imaging

Spectroscopy

Hyperspectral imaging

4 Ds: 2 dimensions of image, wavelength, and time

Biomedical Optical Imaging Laboratory

E-MAIL : [email protected] : 03-4252897TEL : 03-4227151 ext 65276Office : IL-529


  • Login