ToF-SIMS negative ion images of two BLES (bovine lipid extract surfactant)
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ToF-SIMS negative ion images of two BLES (bovine lipid extract surfactant) LB films containing 0 mol% (A, C) and 30 mol% (B, D) cholesterol-d7. M255 corresponds to the palmitate group, M2 to deuterium present in the tail of cholesterol-d7.

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BLES + 30 mol% d7-chol

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Bles 30 mol d7 chol

ToF-SIMS negative ion images of two BLES (bovine lipid extract surfactant)

LB films containing 0 mol% (A, C) and 30 mol% (B, D) cholesterol-d7. M255

corresponds to the palmitate group, M2 to deuterium present in the tail of cholesterol-d7.

Images E, F, G, H were obtained by subjecting A, B, C, D, respectively to a smoothing

function available as part of the IonTof software, and show more clearly the location of

the palmitate group and deuterium. Images are 50 x 50 mm2, 256 x 256 pixels with

1 shot/pixel.

BLES +

30 mol% d7-chol

BLES

A

B

M255

D

C

M2

F

E

M255

processed

G

H

M2

processed

This set of images is published in the Biophysical Journal.  The goal of that work was to determine the function of cholesterol in an animal extracted surfactant (BLES).  TOF-SIMS was used to determine the location of cholesterol in an LB-film of BLES. Courtesy of Eleonora Keating, Univ. of Western Ontario


Bles 30 mol d7 chol

Positive ion TOF-SIMS images of a 50:30:20 d4-DPPC:POPC:d31-POPG LB film deposited on

gold –coated mica at p = 30 mN/m. Mass 50 corresponds to C3D7 which is specific to d31-POPG,

masses 104 and 184 correspond to choline and phosphocholine which are specific to POPC respectively,

masses 108 and 188 correspond to d4-choline and d4-phosphocholine specific to d4-DPPC and mass

739 corresponds to d4-DPPC-H+. These images show that POPC and d31-POPG are present in the LE

phase while d4-DPPC is highly concentrated in the LC phase. Images are 50 x 50 mm2, 256 x 256 pixels

with 1 shot/pixel.

This set of images shows that TOF-SIMS can be used to determine the location of each phospholipid in an LB film of a mixture.  In this work the authors are trying to design a synthetic surfactant that will eventually replace animal extracted BLES which is currently administered to premature babies throughout Canada.

Courtesy of Eleonora Keating, Univ. of Western Ontario


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