1 / 3

1. Stability of PyHA

08-04-2009 Jae Y Lee. 1. Stability of PyHA. Stabilities tests are on-going. Rat cortical astrocyte for 3 days in DMEM/F12K (with 10% FBS, 1% P/S/A) Biotinylated HA binding proteins, followed by PE-streptavidin treatment Cell culture incubator (37’C, 5% CO 2 )

lixue
Download Presentation

1. Stability of PyHA

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. 08-04-2009 Jae Y Lee 1. Stability of PyHA • Stabilities tests are on-going. • Rat cortical astrocyte for 3 days in DMEM/F12K (with 10% FBS, 1% P/S/A) • Biotinylated HA binding proteins, followed by PE-streptavidin treatment • Cell culture incubator (37’C, 5% CO2) • Replaced the solution every other day.

  2. 08-04-2009 Jae Y Lee Stability of PyHA Air – 2 weeks PBS – 3 months Hyase – 2 weeks

  3. 08-04-2009 Jae Y Lee 2. Long-term in vitro astrocyte culture : functional stability in terms of adhesion and migration 10 days 30 days • Previously cultured for 30 days • Fed every other day • 50 µm scale bars • On-going for longer time points 60 days 90 days

More Related