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Human Umbilical Cord Blood Mononuclear Cells And Diabetic Mice (Type I) Norman Ende, MD; Ruifeng Chen, MD; Richard Mack. New Jersey Medical School, Newark, NJ 07003. Preliminary Study

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Human Umbilical Cord Blood Mononuclear Cells And Diabetic Mice (Type I)

Norman Ende, MD; Ruifeng Chen, MD; Richard Mack.

New Jersey Medical School, Newark, NJ 07003

Preliminary Study

Background: Previously we have successfully delayed the onset of vasculites and death in MRL-Lpr/Lpr mice considered to have an autoimmune disease similar to human lupus. Likewise, with the use of megadoses of human umbilical cord blood mononuclear cells, we were able to delay the onset of symptoms and death in SOD1 mice that carry a transgene-for Amyotrophic Lateral Sclerosis also considered by some to be an autoimmune disease. We utilized a similar approach to NOD/LtJ type 1 diabetic mice. No immunosuppression was utilized in this study. It is known that marrow transplantation will ameliorate type I diabetes in mice.

Design: 30 diabetic mice (NOD/LtJ) were divided into 3 groups: A) 10 control mice untreated, B) 10 mice that received congenic bone marrow, 5.6x106 mononuclear cells, C) 10 mice that received 100 x 06 human umbilical cord blood mononuclear cells.

Results: All animals that survived were sacrificed at 137 days of age. At that time in group A, only 4 out of 10 of the control mice were alive with an average blood sugar of 474 mg/dL and an average urine sugar of 607.5 mg/dL. In group B that received congenic marrow cells, 6 out of 10 mice were alive with blood glucose average of 358.8 mg/dL and urine sugar 441mg/dL. In group C, 8 of the 10 mice that received cord blood mononuclear cells were alive at 137 days with an average blood sugar of 239.7 mg/dL and an average urine sugar of 400 mg/dL.

Support by the Abraham S. Ende, Research Foundation.

Final Study

Insulitis

1+ 1+ 2+ 3+ 4+

Conclusion: The survival curve of animals receiving congenic bone marrow and human cord blood mononuclear cell was p< 0.05, p< 0.01 respectively. Final study using 150x106 MNC and 200 x 106 MNC, p<0.01 and p<0.001 respectively. There was a further correlation with glycosuria and the degree of insulitis. This study raises the possibility of utilizing human cord blood mononuclear cells, particularly in conjunction with pancreatic islets, to produce a successful long-term remission in Type I diabetes.

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