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Lab meeting. 2014.02.24 이세라. Gene Scan –B2M. PCR. electrophoresis. Sample 2. Sample 1. 95℃ / 5min. Sequencing-PLK1. Childhood AML -RNA. cDNA 합성. 65 ℃ / 5min -> ice. 2 5 ℃ / 10min -> 50 ℃ / 50min ->85 ℃ / 5min -> ice. 1 μ l of Rnase H. 37 ℃ / 20min -> ice. cDNA ->electrophoresis.

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Presentation Transcript
Lab meeting

Lab meeting

2014.02.24

이세라



electrophoresis

Sample 2.

Sample 1.

95℃ / 5min


Sequencing plk1
Sequencing-PLK1

Childhood AML -RNA


cDNA합성

65 ℃ / 5min -> ice

25 ℃ / 10min -> 50 ℃ / 50min

->85 ℃ / 5min -> ice

1μl of Rnase H

37 ℃ / 20min -> ice


Cdna electrophoresis
cDNA->electrophoresis

cDNA PCR->electrophoresis



Pyrosequencing
pyrosequencing

2014.02.24(BDNF)

2013.09.24(SLC6A4)

PCR전

PCR후


flowcytomety

2014.02.21

M-MDSC

G-MDSC


2/20sample 조일도71411924

M-MDSC

G-MDSC


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