CHEE450: Engineering Biology. IEX Chromatography. Presented by: Nikki Apostolakis Helen So Tiffany Yu. Chromatography Introduction.
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The term “chromatography” applies to a wide range of separation techniques that are based on the differential interaction of molecules between a moving phase and a stationary phase.
Column chromatography is a commonly used technique for protein purification.
Different column chromatography methods exploit different properties of the protein; Ion exchange chromatography separates molecules based on charge.
Resins are the medium for separation and represent the stationary phase. The surface of these particles provide sites for selective binding. Separation is based on reversible adsorption of ions onto resin
Resin Type: Sepharose High Performance
Resin Volume: 207.88 L
Column Type: GE Chromaflow
Inner diameter: 1800 mm
Pressure Rating: 3 bar
BPG BioProcess Columns
Can make batch process continuous by running columns in parallel
Change resin to have impurities bind and allow MAB to pass
Use a different basis for separation (e.g. molecular size)
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