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Preliminary Full Crew Meeting: Year 2 - PowerPoint PPT Presentation


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Background Demographic: Undergraduate Students in Pre-Lab Project: Streaming On-line Biology Video pieces: One per Preceptor, per year. Individual Video Breakdown: Intro: Host, Dr. Robert Lue Faculty Spotlight: Harvard Professor in the particular field of lab and study

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Background

Demographic:

  • Undergraduate Students in Pre-Lab

    Project:

  • Streaming On-line Biology Video pieces: One per Preceptor, per year.

  • Individual Video Breakdown:

  • Intro: Host, Dr. Robert Lue

  • Faculty Spotlight: Harvard Professor in the particular field of lab and study

  • Lab/Pre-Lab: Actual Hands On Demonstration of Lab of Topic

  • Outro: Host, Dr. Robert Lue

  • Mandatory Feed Back Form (Quiz)

  • Browser/Monitor:

  • Mac/PC; Quicktime/Flash


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“Players”

Executive Producer:

  • Robert Lue 5-9924

    Associate Producer:

  • Alain Viel 6-5606

    Director of Photography/Production Manager:

  • Leigh Stimolo 6-7093

    Content Supervisor/Coordinator:

  • Christine Rodriguez 203-665-6434

    Writers:

  • Cheryl Vaughan 401-383-5968

  • Christine Rodriguez 203-665-6434

  • Tom Torello 6-1206

  • Erica Pantages 4-7925

  • Marion Shonn TBA

  • On-Location Audio Engineer:

  • Jack Howard 5-4035

Videographers/Editors:

  • Lansing Wagner 5-2683

  • Steve Keirstead 5-2683

  • Leigh Stimolo 6-7093

    Production Assistants:

  • Eliza Stephens 5-8258

  • Clark Magnan 5-8258

    Voice Over Studio/Engineer:

  • Bill Countie, Media Production Center 5-9440

    Animator:

  • Matt Bohan, Freelance Flash Animator

  • A/V Multimedia Developer:

  • Leigh Stimolo 6-7093

    Video Server Network Administrators:

  • Webmaster: Steve Keirstead 5-2683

  • Chiasm Server: Pepe Lopez 6-8479


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Pre-production Deliverables

Choose Lab Topic

Create Outline

Storyboard Lab Video Sequence

Script Lab Video Sequence

Begin Location Scouting

Research and Submit Talent Selection

Script Rewrite

Talent Rehearsals

Shot List Breakdown

Book Location

Book Talent

Book Crew

Recruit Production Assistants

Finalized Script with Shot Sequence

1-2 Day Lab Shoot

Collect Signed Talent Release Forms

Collect Master Tapes & Label

Hand off Master Lab Video Tapes to Editor

Select Faculty member for Spotlight


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Pre-production Deliverables

Compose Ten Faculty Spotlight Questions

Submit Questions

Optional Presence at Faculty Spotlight Shoot

Compose a small 1-2 paragraph Intro and Outro script

Compose Evaluation Questions for Quiz


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Production Deliverables

Lab Video Sequence Shoot—

Audio: Jack Howard

Video: Steve Keirstead (BS 54)

and Lansing Wagner (BS 50/BS 52)

Lighting: Leigh Stimolo

Faculty Spotlight Shoot—

Audio: Jack Howard

Video: Leigh Stimolo

Lighting: Leigh Stimolo

Interviewer: Robert Lue

Intro/Outro Shoot—

Audio: Jack Howard

Video: Leigh Stimolo

Lighting: Leigh Stimolo


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Post-Production Deliverables

Animation Sequences—

Animation Script

Animation Storyboard

Animator: Matt Bohan

Lab Video Sequence Editing—

Video Editors: Lansing Wagner (BS 50 & BS 52) and Steve Keirstead (BS 54)

Line Producer: Leigh Stimolo

On-line Compressionist: Leigh Stimolo

Voice Over (VO)—

Voice Over Script

VO Studio: Bill Countie, Media Production Center

User Interface—

Developer: Leigh Stimolo

Web site—

Developer/Programmer: Leigh Stimolo


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BS 50: Topics

BS50

  • Sterile techniques (basics, plating microorganisms, titers and dilutions, mammalian cell culture)

  • Chip analysis (techniques, SNPs/pharmacogenomics, transcriptional profiling, limitations of data)

  • Meiosis and tetrad analysis (Meiosis animation, Neurospora vs Saccharomyces, tetrad dissection vs random sporulation)

  • Use of reporters (B-galactosidase, GFP, others)

  • Northerns and Southerns

  • Cloning, transformation, and transfection


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BS 52: Topics

BS 52

  • Electrophoresis (agarose vs acrylamide, what %, what Amp/Volt to run at, IEF, capillary)

  • DNA sequencing (Restriction enzyme mapping, lab/gel techniques, methods such as PCR, Sanger, automated, etc.)

  • DNA topology (1D/2D gels, animation of enzymatic reaction, run on gel)

  • Gel shift/analysis of DNA binding proteins

  • Staining and labeling (histology/cells, DNA, types of stains used, when and why they work, radioactivity, enzymatic assays)

  • FISHhttp://www.courses.fas.harvard.edu/~bs52


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BS 54: Topics

BS54

  • Centrifugation (vocabulary, techniques, types of rotors, supernatant vs pellet, from microfuge to Sorvall to ultra)

  • Generation of antibodies (polyclonal vs monoclonal, modified secondary antibodies)

  • Beads and Tags (biochemical sorting, IP/Co-IPs, GST, 6-His, cell sorting, agarose beads, etc.)

  • Gradients (refer to ultracentrifugation, subcellular fractionation, how to get molecular materials, labelled organelles?, DNA/CsCl gradient, proteins such as ribosomes, etc)

  • Protein sequencing and analysis (old vs new methods)

  • ELISAhttp://www.courses.fas.harvard.edu/~bs54


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BS 50: Year 1

BS50 : Preceptor, Christine Rodriguez (Rich Dearborn, Adam Fagen)

  • MICROARRAY, Chip Analysis

    On-Screen Pre-Lab Talent:

  • Claire Bailey

    Voice Over Talent:

  • Claire Bailey (NYR)

    Faculty Spotlight:

  • TBD, possibles: Michael Laub, Hans Hoffman (NYR)

    Other Content and Production Assistants:

  • Undergraduate Student: Joo Hee & Allison


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BS 52: Year 1

BS52 : Preceptor, Kenna Mills (Lauraine Dalton)

  • DNA SEQUENCING

    On-Screen Pre-Lab Talent:

  • Dion Dickman

    Voice Over Talent:

  • Jan or Dion Dickman (NYR)

    Faculty Spotlight:

  • Dan Branton

    Other Content and Production Assistants:

  • Undergraduate Student: N/A


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BS 54: Year 1

BS54: Preceptor, Cheryl Vaughan

  • ANALYZING PROTEINS

    On-Screen Pre-Lab Talent:

  • John Neven, Emy Chen, Darcy Mootz

    Voice Over Talent:

  • Darcy Mootz

    Faculty Spotlight:

  • TBD, possibles: Steve Gygi (NYR)

    Other Content and Production Assistants:

  • Undergraduate Student: N/A


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BS 50: Year 2

BS50 : Preceptor, Christine Rodriguez (Erica Pantages)

  • NORTHERN/SOUTHERN, Cloning

    On-Screen Pre-Lab Talent:

  • TBA (NYR)

    Voice Over Talent:

  • TBA (NYR)

    Faculty Spotlight:

  • TBD, possibles: TBA (NYR)

    Other Content and Production Assistants:

  • Undergraduate Student: TBA


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BS 52: Year 2

BS52 : Preceptor, Tom Torello

  • TBD

    On-Screen Pre-Lab Talent:

  • TBA (NYR)

    Voice Over Talent:

  • TBA (NYR)

    Faculty Spotlight:

  • TBD, possibles: TBA (NYR)

    Other Content and Production Assistants:

  • Undergraduate Student: TBA


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BS 54: Year 2

BS54: Preceptor, Cheryl Vaughan (Marion Shonn)

  • ANTIBODIES

    On-Screen Pre-Lab Talent:

  • TBA (NYR)

    Voice Over Talent:

  • TBA (NYR)

    Faculty Spotlight:

  • TBD, possibles: TBA (NYR)

    Other Content and Production Assistants:

  • Undergraduate Student: TBA


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YEAR TWO emails

Robert Lue [email protected]

Leigh Stimolo [email protected]

Jack Howard [email protected]

Alain Viel [email protected]

Cheryl Vaughan [email protected]

Christine Rodriguez [email protected]

Lansing Wagner [email protected]

Steve Keirstead [email protected]

Kenna Mills [email protected]

Tom Torello [email protected]

Erica Pantages [email protected]

Marion Shonn [email protected]

Eliza Stephens [email protected]

Clark Magnan [email protected]


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Equipment: Camera/Audio

Shotgun Microphone

Fishpole

Canon XL-1S Mini DV Camcorder

FP-33 Stereo Mixer

Lavalier Microphone


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Equipment: Lights

Arri: 300 Watt Fresnel

Tungsten Light

Tota Light Kit with

Umbrellas & Stands

SoftBox (Chimera)


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