13er Congreso Colombiano de Psicología

1. 13er Congreso Colombiano de Psicología Different patterns of defensive freezing response associated with different anxiety disorders Jesús Landeira-Fernandez

2. Functions of the Nervous System Two functions of the nervous system Regulation of the internal environment through homeostasis Visceral Nervous System Interact with external environment through behavior Somatic Nervous System

3. Organization of the Nervous System STIMULUS BEHAVIOR Defense Feeding Reproduction

4. Organization of the Defense System Dangerous Stimulus Nervous System Defensive Response

5. Organization of the Defense System Dangerous Stimulus Innate Cause some threat to the life to the individual Learned

6. Organization of the Defense System

7. Organization of the Defense System Danger Stimulus Behavioral Inhibition Freezing Escape Defensive Response Fight Behavioral Activation ACTIVITY BURST

8. Organization of the Defense System Theoretical Constructs

9. Organization of the Defense System Somatic Nervous System Visceral Nervous System

10. Organization of the Defense System Somatic Nervous System Visceral Nervous System

11. Interaction between Pain and Fear Motivational System Pain motivational system activates fear motivational system through classical conditioning Pain Motivational System Fear Motivational System Contextual Fear Conditioning Neutral Stimuli

12. Interaction between Fear and Fear Motivational System Fear motivational system inhibits pain motivational system through analgesia Fear Motivational System Pain Motivational System Analgesia

13. Interaction between Fear and Fear Motivational System Classical Conditioning Analgesia

14. Contextual Fear Conditioning Paradigm First Day 0:00 3:00 3:20 3:40 4:00 A few minutes of habituation Dia 1 Treinamento A few rapid and not very intense footshocks 0:00 3:00 Second Day: Testing Session

15. Typical Contextual fear Conditioning Result Freezing depends on the intensity of the shock

16. Activation of the Pain Motivational System in the Vacuum: The Immediate Shock Deficit

17. Activation of the fear Motivational System in the Vacuum: The Immediate Shock Deficit

18. Delayed but not Immediate Shock Induces Analgesia

19. Immediate Shock does not Induce Analgesia

20. Opioid Analgesia

21. Opioid Analgesia

22. Non-Opioid Analgesia

23. Fear and Pain Motivational Systems Dangerous Stimuli Motivational Systems Defense Responses Distal Innate Fear Freezing Learned Innate Classical Conditioning Analgesia Pain Activity Burst Proximal

24. Fear and Pain x Anxiety and Panic Motivational Systems Defense Responses Motivational Systems Dysfunctions Fear Anxiety Freezing Classical Conditioning Analgesia Panic Attack Pain Activity Burst

25. Deakin-Graeff Model Dysfunctions Anxiety Activates Inhibits Panic Attack

26. Functions x Dysfunctions Fanselow Model Deakin-Graeff Model Functions Dysfunctions Fear Anxiety Activates Classical Conditioning Inhibits Analgesia Panic Attack Pain

27. Animal Models to Investigate Anxiety and Panic Attack Contextual fear conditioning (Model of Generalized Anxiety Disorder) dPAG Electrical Stimulation (Model of Panic Attack)

28. Animal Model of Panic Attack dPAG electrical stimulation

29. dPAG electrical stimulation Increasing the intensity of the dPAG electrical stimulation there is first a freezing response followed by vigorous running and jump reactions.

30. The Periaqueductal Gray Matter Periaqueductal Gray (PAG) Electrode Tract Stimulation Site in the Dorsal Portion of the PAG Cerebral Aqueduct

31. Does Anxiety (contextual fear conditioning) Inhibits Panic Attacks (dPAG electrical stimulation)? dPAG Electrical Stimulation (Model of Panic Attack) Contextual fear conditioning (Model of Generalized Anxiety Disorder)

32. Does Anxiety (contextual fear conditioning) Inhibits Panic Attacks (dPAG electrical stimulation)?

33. Two Types of Freezing: the context shift test Contextual fear conditioning

34. Two Types of Freezing: the context shift test Contextual fear conditioning

35. Two Types of Freezing: the context shift test dPAG electrical stimulation

36. Two Types of Freezing: the context shift test dPAG electrical stimulation

37. Two Types of Freezing: the context shift test Contextual fear conditioning dPAG electrical stimulation

38. Two Types of Freezing Freezing Induced by Contextual Fear Conditining Amygdala vPAG Freezing Freezing Induced by dPAG Electrical Stimulation ? dPAG ?

39. Comparison between dlPAG and vlPAG electrical stimulation

40. Comparison between dlPAG and vlPAG electrical stimulation Freezing after dPAG electrical stimulation

41. Effect of vPAG Lesions on Conditioned and Unconditioned Freezing

42. Histology of vPAG Lesion and dPAG Electrical Stimulation vPAG Lesions dPAG Electrical Stimulation

43. Effect of vPAG Lesions on Conditioned and Unconditioned Freezing Contextual fear conditioning dPAG electrical stimulation

44. Effect of Amydgala Lesions on Conditioned and Unconditioned Freezing

45. Histology of Amygdala Lesion and dPAG Electrical Stimulation dPAG Stimulation Amygdala Lesions

46. Effect of Amygdala Lesions on Conditioned and Unconditioned Freezing Contextual fear conditioning dPAG electrical stimulation

47. Summary

48. Amygdala vPAG Freezing ? dPAG ? Summary Fear Motivational System Responsibe for General Anxiety Disorder Pain Motivational System Responsibe for Panic Attack

49. Collaborators Marcus Brandão – USP-RP A.Pedro de Mello Cruz - UnB Regina Nogueira - UNESA Daniel M Vianna – USP-RP Valeska Mageriek - UNIFESP Cecília De Luca – USP-RP Luciana Oliveira – USP-RP Vitor Castro – PUC-Rio Bruno Galvão – PUC-Rio Bruno Larrubia – PUC-Rio

50. Contact www.landeira.org landeira@puc-rio.br Thanks to Fernando Cárdenas Universidad de los Andes Muchas Grácias !!!