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Blood Typing Lab

Blood Typing Lab. Whodunit?. Blood typing. What’s your blood type? O+ ABO and Rh are totally independent of each other. ABO Antigens and Antibodies. The Rh Factor. Also called D antigen Either Rh positive (Rh + ) or Rh negative (Rh - )

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Blood Typing Lab

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  1. Blood Typing Lab Whodunit?

  2. Blood typing • What’s your blood type? O+ • ABO and Rh are totally independent of each other

  3. ABO Antigens and Antibodies

  4. The Rh Factor • Also called D antigen • Either Rh positive (Rh+) or Rh negative (Rh-) • Only sensitized Rh- blood has anti-Rh antibodies

  5. Blood Types • Genetically determined cell surface markers (antigens) on RBCs, including • ABO group – glycolipids on RBC surface • Rh factor – membrane protein

  6. 4 Basic Blood Types • A has surface antigen A • B has surface antigen B • AB has both antigens A and B • O has neither A nor B • A has type B antibodies • B has type A antibodies • O has both A and B antibodies • AB has neither A nor B antibodies

  7. 4 Basic Blood Types • Antigens also called agglutinogens • Antibodies called agglutinins Figure 19–6a

  8. ABO Antigens and Antibodies

  9. The Rh Factor • Also called D antigen • Either Rh positive (Rh+) or Rh negative (Rh-) • Only sensitized Rh- blood has anti-Rh antibodies

  10. Blood type questions • Which blood type is the best in emergency settings (hint: which type can be given to anyone?) • Which blood type is the lucky one that can receive blood from any donor?

  11. Cross-Reaction Figure 19–6b

  12. Cross-Reaction • If donor and recipient blood types not compatible: • Plasma antibody meets its specific surface antigen and blood will agglutinate and hemolyze

  13. Blood Type Test • Determines blood type and compatibility Figure 19–7

  14. Cross-Match Test • Performed on donor and recipient blood for compatibility to blood surface antigens other than ABO and Rh

  15. Blood typing procedures • Skip part A • Part B: 6 trays with three wells each Victim Crime scene Suspect 1 A B Rh A B Rh A B Rh Suspect 2 Suspect 3 Suspect 4 A B Rh A B Rh A B Rh 6 Bottles of “blood” 3 Bottles of antibodies

  16. Blood Typing Procedures • For each well Place 1-2 drops of blood from one bottle in all 3 wells • 1-2 drops of crime scene in each of the three wells • 1-2 drops of victim in each of the three wells • 1-2 drops of suspect 1 in each of the three wells • 1-2 drops of suspect 2 in each of the three wells • 1-2 drops of suspect 3 in each of the three wells • 1-2 drops of suspect 4 in in each of the three wells • For each tray add: • 1-3 drops of anti-A serum to one well and stir • 1-3 drops of anti-B serum to one well and stir • 1-3 drops of anti-Rh serum to one well and stir Look for clumping reaction

  17. Blood type? • What is the blood type? x = clumping rxn. • Next question: what antibodies does this person have in their blood? A B Rh X X

  18. To turn in • Lab report pages 5 and 6 with completed table and questions • Due next Thursday

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