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Antigen-Antibody Interactions. Dr. Conrad Sept. 10,2008. Antigenicity. Requirements to be an immunogen Low mw antigens (haptens) Adjuvants Primary vs secondary responses. Concept of a Hapten. r/c = Kn-Kr. c = concentration of free antigen (hapten) K = K a = equilibrium constant

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Antigen antibody interactions

Antigen-Antibody Interactions

Dr. Conrad

Sept. 10,2008


Antigenicity
Antigenicity

  • Requirements to be an immunogen

  • Low mw antigens (haptens)

  • Adjuvants

  • Primary vs secondary responses



r/c = Kn-Kr

c = concentration of free antigen (hapten)

K = Ka = equilibrium constant

r = hapten bound per unit Ab

n = Valence of Ab




Factors which influence ab affinity
Factors which influence Ab affinity

  • Temp, pH, ionic strength

  • Polyclonal vs monoclonal

  • Ab class

  • Type of antigen






Comparison of Hv sequences of monoclonals binding the

Same vs binding a different antigen epitope


Affinity vs avidity – depends on valence

Avidity – sum total or strength of binding of two molecules (or cells) to one another at multiple sites. Distinct from affinity which is the strength of binding of one site on a molecule to its ligand.






ELISA

RIA







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