Rhizome Project Meeting(2) 5-4-2011. Min- Jeong Kim Gang Lab Institute of Biological Chemistry Washington State University Pullman, WA. Why Johnsongrass ?. Johnsongrass. www.usda.gov. Distribution of Johnsongrass. www.usda.gov. Work Flow. Total RNA. cDNA synthesis.
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Institute of Biological Chemistry
Washington State University
PCR with gene-specific primers
LR rxn with pK7GWIWG2(II)
Cloning into pCR8/GW/TOPO vector
Transfer into LBA4404 Agro. strain
Alignment of Knox genes in Sorghum bicolor (3)
Karimi, M., Inzé, D., Depicker, A., Gateway vectors for Agrobacterium-mediated plant transformation. Trends Plant Sci. 2002 May;7(5): 193-195
M, marker; PC, positive control (vector); NT, non-transgenic
M, size marker; PC, positive control (vector); NT, non-transgenic;
NC, negative control (without PCR template)