Rhizome project meeting 2 5 4 2011
This presentation is the property of its rightful owner.
Sponsored Links
1 / 22

Rhizome Project Meeting(2) 5-4-2011 PowerPoint PPT Presentation


  • 44 Views
  • Uploaded on
  • Presentation posted in: General

Rhizome Project Meeting(2) 5-4-2011. Min- Jeong Kim Gang Lab Institute of Biological Chemistry Washington State University Pullman, WA. Why Johnsongrass ?. Johnsongrass. www.usda.gov. Distribution of Johnsongrass. www.usda.gov. Work Flow. Total RNA. cDNA synthesis.

Download Presentation

Rhizome Project Meeting(2) 5-4-2011

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -

Presentation Transcript


Rhizome project meeting 2 5 4 2011

Rhizome Project Meeting(2)5-4-2011

Min-Jeong Kim

Gang Lab

Institute of Biological Chemistry

Washington State University

Pullman, WA


Rhizome project meeting 2 5 4 2011

Why Johnsongrass?


Rhizome project meeting 2 5 4 2011

Johnsongrass

www.usda.gov


Distribution of johnsongrass

Distribution of Johnsongrass

www.usda.gov


Rhizome project meeting 2 5 4 2011

Work Flow

Total RNA

cDNA synthesis

PCR with gene-specific primers

LR rxn with pK7GWIWG2(II)

Sequence confirmation

Cloning into pCR8/GW/TOPO vector

Sequence confirmation

Transfer into LBA4404 Agro. strain

Plant transformation


Rhizome project meeting 2 5 4 2011

0 week

1 week

2 weeks

4 weeks


Expression analysis of knox transcription factors based on ginger and turmeric microarray data

Expression Analysis ofKnox Transcription Factors(Based on Ginger and Turmeric Microarray Data)


Knox 1

Knox (1)

Intensity


Knox 2

Knox (2)

Intensity


Knox 3

Knox (3)

Intensity


Knox 4

Knox (4)

Intensity


Knox 5

Knox (5)

Intensity


Alignment of knox genes in sorghum bicolor 1

Alignment of Knox genes in Sorghum bicolor (1)


Alignment of knox genes in sorghum bicolor 2

Alignment of Knox genes in Sorghum bicolor (2)


Rhizome project meeting 2 5 4 2011

Alignment of Knox genes in Sorghum bicolor (3)


Alignment of knox genes in sorghum bicolor 4

Alignment of Knox genes in Sorghum bicolor (4)


Map of pk7gwiwg2 ii rnai vector

Map of pK7GWIWG2(II) RNAi Vector

Karimi, M., Inzé, D., Depicker, A., Gateway vectors for Agrobacterium-mediated plant transformation. Trends Plant Sci. 2002 May;7(5): 193-195


Transgenic plants on selection media

Transgenic Plants on Selection Media


Transgenic plants in the greenhouse

Transgenic Plants in the Greenhouse


Insert confirmation 1

Insert Confirmation (1)

M

PC

NT

2

6

10

M

M, marker; PC, positive control (vector); NT, non-transgenic


Insert confirmation 2

Insert Confirmation (2)

M

PC

NT

2

6

10

12

16

M

NC

M, size marker; PC, positive control (vector); NT, non-transgenic;

NC, negative control (without PCR template)


Thank you

Thank You


  • Login