slide1
Download
Skip this Video
Download Presentation
stable δ N 15 ratios help differentiate nitrogen sources

Loading in 2 Seconds...

play fullscreen
1 / 22

stable δ N 15 ratios help differentiate nitrogen sources - PowerPoint PPT Presentation


  • 45 Views
  • Uploaded on

Using stable δ N 15 ratios to help differentiate nitrogen sources within a residential community- another tool for the TMDL toolbox.

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about ' stable δ N 15 ratios help differentiate nitrogen sources' - leo-wilson


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
slide1
Using stable δN15 ratios to help differentiate nitrogen sources within a residential community- another tool for the TMDL toolbox.
  • Nora Egan Demers Associate Professor of Biology and Interdisciplinary StudiesFlorida Gulf Coast UniversityFt. Myers, Florida
  • Report to the Watershed Council
  • January 17, 2008
stable n 15 ratios help differentiate nitrogen sources
Ratio is based on heavy and light form of N

In general – according to the literature

δN15between 2.00 and 8 is from atmospheric

δN15between -4.00 and 4.00 is from fertilizer

δN15between 18.00 and 22.00 is from humans

δ15N =[R(sample)-R(standard)/R(standard)]*1000

where R= ratio of 15N/14N and R standard is the atmospheric standard.

Generally, Nitrogen from fertilizers has an δ15N around 0+- 4 0/00 (parts per thousand) (Heaton, 1986), while Nitrogen from septic systems and animal waste has a δ15N from +8 to +22 0/00. (Heaton, 1986). Levels of δ 15N in atmospheric depositions are about +2 to +8.00 0/00 (Kreitler et al, 1978).

This is because the lighter 14N isotope is preferentially fractionated during bacterial nitrification, resulting in higher δ15N ratios as you move toward meat-eating consumers in the food web.

stable δN15 ratios help differentiate nitrogen sources
lee county utilities overlay
Lee County Utilities overlay

showing sewer and water infrastructure for San Carlos Park, Lee County, Florida. Areas in green are served by municipal sewer and water, in blue by water, (with septic) and in red have well-water and septic systems.

winged foot oriole
Winged Foot/Oriole

(mg/L) 3/07 9/07

  • Ammonia 0.871 0.453
  • Nitrite 0.008 0.01
  • Nitrate 0.01 0.01
  • PO4 0.09 0.09
  • TKN 62 5.9
  • TN 62 5.9
  • Total PO4 8.3 .59
winged foot oriole1
Winged Foot/Oriole

Delta N 15

  • Duckweed (March) 0.86, 3.67
  • Duckweed (Sept) 3.13 2.83
  • Extract flock (Sept) 1.87
kakareka
Kakareka

(mg/L) March Sept

  • Ammonia 0.222 0.049
  • Nitrite 0.008 0.004
  • Nitrate 0.01 0.02
  • PO4 0 0
  • TKN 1.1 0.94
  • TN 1.12 0.96
  • Total PO4 0.046 0.027
kakareka1
Kakareka

Delta N 15

  • Sediments leafy (March) 1.70
  • Algae (March) 2.66
  • Sediment flock (March) 2.50 2.38
  • Roots (Sept) -0.90 -1.82
  • Scrapings from leaves (Sept) -0.35
laurel valley new jersey
Laurel Valley/New Jersey

(mg/L) Sept

  • Ammonia 0.348
  • Nitrite 0.007
  • Nitrate 0.04
  • PO4 0
  • TKN 1.1
  • TN 1.1
  • Total PO4 0.022
laurel valley new jersey1
Laurel Valley/New Jersey

Delta N 15 (Sept)

Flock 5.07

Lettuce leaves 0.93

Lettuce roots -0.98

Extract flock 5.62

demers
Demers

(mg/L) March Sept

  • Ammonia 0.042 0.315
  • Nitrite 0.007 0.014
  • Nitrate 0.02 0.06
  • PO4 0.02 0
  • TKN 1.8 0.94
  • TN 1.83 1
  • Total PO4 0.17 .0033
demers1
Demers

Sept.

Floating blob: 4.68 6.02

Lettuce leaf 2.92 3.82

Lettuce roots 4.47

Sediment flock 5.79

Extract flock 4.34 4.95 2.92

Delta N 15

March

floaty algae 4.08 leafy 4.15

Sediment 6.49 5.9

constitution
Constitution

(mg/L) March Sept

  • Ammonia 0.087 0.052
  • Nitrite 0.008 0.006
  • Nitrate 0.15 0.21
  • PO4 0.01 0
  • TKN 0.81 0.58
  • TN 0.97 0.8
  • Total PO4 0.084 0.018
constitution1
Constitution

Delta N 15

Macroalgae (March) 8.72

Sediment flock (March) 6.39

Rinsed roots (Sept) 9.27

Sediment flock (Sept) 4.24

Extract flock (Sept) 5.37 5.32

outfall 46b 9gr
Outfall: 46B-9GR

( mg/L) March Sept. Ammonia 0.067 0.017

  • Nitrite 0.008 0.004
  • Nitrate 0.2 0.22
  • PO4 0.01 0
  • TKN 0.57 0.83
  • TN 0.78 1.1
  • Total PO4 0.026 .009
outfall 46b 9gr1
Outfall: 46B-9GR

Delta N 15

Green algae (March) 11.05

Brown macro algae (March) 8.51

Sediment flock (March) 10.65

Sept:

Root ball rinsed 6.03

Algae 7.43

Sediment flock 5.77

Extract flock 7.35

golf course effluent
Golf Course Effluent

(mg/L) March Sept

  • Ammonia 0.414 0.213
  • Nitrite 0.178 0.098
  • Nitrate 0.44 2.48
  • PO4 0.67 .73
  • TKN 2.4 2.3
  • TN 3.02 4.8
  • Total PO4 1.00 0.89

Average of 200,000 gallons per day of reclaimed water delivered to this lake

golf course effluent1
Golf Course Effluent

Delta N 15

Sediment Flock

March 17.84

Sept 17.80 19.46 17.68 16.79

golf course lake
Golf Course- Lake

(mg/L) March Sept

  • Ammonia 0.2 0.105
  • Nitrite 0.009 0.006
  • Nitrate 0.01 0.09
  • PO4 0.02 0
  • TKN 1.8 2
  • TN 1.82 2.1
  • Total PO4 0.13 0.13
golf course lake1
Golf Course- Lake

Delta N 15

March Sediment Flock 9.96

Sept

scraping off leaves 6.98 5.05

Sediment flock 7.81

Extract flock 7.69

ad