Brief Review of DNA Replication:
This presentation is the property of its rightful owner.
Sponsored Links
1 / 11

Brief Review of DNA Replication: PowerPoint PPT Presentation


  • 42 Views
  • Uploaded on
  • Presentation posted in: General

Brief Review of DNA Replication:. Nearest Neighbor Analysis (Kornberg 1956):. In vitro DNA synthesis •Template DNA •DNA polymerase •dNTPs. *dATP. *dCTP. *dGTP. *dTTP. •Allow DNA replication to take place •Denature DNA •Treat with micrococcal nuclease-

Download Presentation

Brief Review of DNA Replication:

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -

Presentation Transcript


Brief review of dna replication

Brief Review of DNA Replication:


Brief review of dna replication

Nearest Neighbor Analysis (Kornberg 1956):

In vitro DNA synthesis

•Template DNA

•DNA polymerase

•dNTPs

*dATP

*dCTP

*dGTP

*dTTP

•Allow DNA replication to take place

•Denature DNA

•Treat with micrococcal nuclease-

yields mononucleotides with 3’ terminal phosphates


Brief review of dna replication

ssDNA treated with micrococcal nuclease:

A

A

C

C

C

*

G

G

T

T

T

T

C

ssDNA:

*


Brief review of dna replication

Exercise:

List all possible dinucleotides (analysis of nearest 5’ neighbor)

Which must be present in equal frequencies if the DNA strands are:

Parallel?Antiparallel?


Brief review of dna replication

ApA

ApC

ApG

ApT

CpA

CpC

CpG

CpT

GpA

GpC

GpG

GpT

TpA

TpC

TpG

TpT

Exercise:

List all possible dinucleotides (analysis of nearest 5’ neighbor)

Which must be present in equal frequencies if the DNA strands are:

Parallel?Antiparallel?

ApC = TpGCpA = GpT

ApG = TpCCpG = GpC

ApT = TpACpT = GpA

ApC = GpTCpA = TpG

ApG = CpTGpA = TpC


Brief review of dna replication

Alternative Forms of DNA


Brief review of dna replication

G + C Content


Dna melting

DNA Melting

*Temperature at which DNA strands are ½ denatured is the melting temperature or Tm

*GC content of DNA has a significant effect on Tm


Brief review of dna replication

Comparison of Typical Prokaryotic and Eukaryotic Cells


Brief review of dna replication

Chromosome Sequence and Diversity


Brief review of dna replication

Gene Density in Different Organisms


  • Login