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Engineering BioBrick vectors from BioBrick parts

Engineering BioBrick vectors from BioBrick parts. Reshma P Shetty , Drew Endy and Thomas F Knight Jr. Outline. Background Synthetic Biology Terms Method Current Research Discussion. Synthetic Biology. What is Synthetic Biology? A Method Milestones Recombinant DNA

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Engineering BioBrick vectors from BioBrick parts

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  1. Engineering BioBrick vectors from BioBrick parts Reshma P Shetty, Drew Endy and Thomas F Knight Jr

  2. Outline • Background • Synthetic Biology • Terms • Method • Current Research • Discussion

  3. Synthetic Biology • What is Synthetic Biology? • A Method • Milestones • Recombinant DNA • PCR(Polymerase Chain Reaction) • Automated Sequencing • Automated Construction • Standards • Abstractions -Write Last 30 years -------------------Read Synthetic Biology

  4. Synthetic Biology (Cont.) • Systems • Devices • Parts • DNA

  5. Terms • Vector • Plasmid • Polymerase • Restriction enzyme • Ligase

  6. Method • Goal • Standardize the BioBrick vectors’ assembly process • Maintain • Cloning site • Selection marker • High copy orgin • Terminators flanking the cloning site • Verification primer annealing sites • Process • Build new BioBrick vectors’ backbone • Standard assembly for new BioBrick

  7. Method(Cont.) • Basic BioBrick

  8. Method(Cont.) • Build new BioBrick vectors’ backbone

  9. Method(Cont.) • Standard assembly for new BioBrick

  10. Method(Cont.) • Check • Cloning site • Selection marker • High copy orgin • Terminators flanking the cloning site • Verification primer annealing sites

  11. Current Research • More than 200 parts • Some Systems

  12. Discussion • Wetware • Biological film • Digital counting machine • Interdisciplinary research • Computer aided design

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