DNA
Download
1 / 50

DNA - PowerPoint PPT Presentation


  • 57 Views
  • Uploaded on

DNA. Chapter 13. What is DNA?. DNA Structure. DNA stands for deoxyribonucleic acid Long molecule made up of nucleotides Consists of a 5- carbon sugar, phosphate group, and a nitrogenous base (adenine, guanine, cytosine, and thymine) Backbone of DNA is sugar and phosphate

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about ' DNA' - lavey


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript

DNA

Chapter 13



Dna structure
DNA Structure

  • DNA stands for deoxyribonucleic acid

  • Long molecule made up of nucleotides

    • Consists of a 5- carbon sugar, phosphate group, and a nitrogenous base (adenine, guanine, cytosine, and thymine)

  • Backbone of DNA is sugar and phosphate

    • Nitrogenous bases stick out

SUGAR

Phosphate

SUGAR

Phosphate

SUGAR

Phosphate

SUGAR

A

G

C

T


DNA

  • Is found in all nucleated body cells

    • White blood cells

    • Semen

    • Saliva

    • Urine

    • Hair

    • Root

    • Teeth

    • Bone

    • Tissue

  • Is most abundant in buccal (cheek) cells


Chromosomes
Chromosomes

  • Composed of tightly coiled DNA and proteins

  • Humans have 46 chromosomes

  • Only visible during cell division

  • Consists of 2 arms called chromatids held together by the centromere


Genes
Genes

  • Are portions of DNA that code for specific proteins

  • Is the fundamental unit of heredity

    • Each gene(s) codes for specific traits

      • Eye color, hair color, height, etc



James watson and francis crick
James Watson and Francis Crick

  • Determined that DNA is a double helix


Historical milestones
Historical Milestones

  • 1988

    • FBI starts DNA casework

  • 1991

    • First STR (short tandem repeat) paper

  • 1998

    • FBI launches CODIS database



Complementary base pairing
Complementary Base Pairing

  • Is based on Chargaff’s rules

    • Adenine pairs with Thymine

    • Cytosine pairs with Guanine

  • Ex:) ATTGCGTAGGGCTA (TEMPLATE)

    TAACGCATCCCGAT (COMPLEMENT)


Dna replication
DNA Replication

  • Before a cell divides, it duplicates its DNA

    • Process called replication

  • Process involves the DNA molecule separating into 2 strands and then producing two new complementary strands

    • Each strand of the DNA acts as a “template”

  • Occurs until each chromosome is copied


Dna replication1
DNA Replication

  • Is carried out by enzymes that “unzip” the DNA molecule

    • Occurs when hydrogen bonds between the base pairs are broken

  • Principle enzyme involved is DNA polymerase

    • Polymerizes nucleotides to produce DNA

    • Also proofreads each DNA strand


RNA

  • Is transcribed from DNA through the process of transcription

    • A goes with U instead of T

    • G goes with C

  • After transcription comes translation

    • Pairing up of codons with anticodons on tRNA to form an amino acid


Genetic code
Genetic Code

  • Proteins made by joining amino acids into chains called polypeptides

  • There are twenty amino acids

    • Properties of proteins determined by the order in which these amino acids are in

  • Language of mRNA instructions called genetic code


Genetic code1
Genetic Code

  • RNA has four bases- A, U, G, C

  • Genetic code read in threes

    • Called a codon

    • Each three letters represents a specific amino acid

      • Can be specified by more than one codon



Dna typing1
DNA Typing

  • Is a method in which DNA is converted into a series of bands that ultimately distinguish each individual

    • Involves repeating segments of DNA numerous times

      • Called tandem repeats

        • Act as fillers or spaces between the coding regions of DNA

  • Only 1/10th of a single percent of DNA differs from one person to the next

    • Used to generate DNA profiles of an individual


Dna typing2
DNA Typing

  • Has coding and non-coding regions

    • 3% of the human DNA sequences code for proteins

    • 97% is non-coding and is repetitive

      • Same sequences repeated over and over

      • Are the tandem repeats


Dna typing3
DNA Typing

  • 3 processes utilized

    • RFLP

      • Restriction Fragment Length Polymorphism

    • PCR

      • Polymerase Chain Reaction

    • STR

      • Short Tandem Repeats


RFLP

  • Restriction enzymes are used to cut DNA into smaller fragments that can be separated and characterized for identification

  • Steps involved

    • Isolate

      • Separate DNA from the cell

    • Cut

      • Using restriction enzymes to make shorter base strands

    • Sort

      • By size using electrophoresis

    • Analyze

      • The specific alleles for identification


RFLP

  • To visualize the RFLP’s, the fragments are transferred to a nylon membrane that has been treated with radioactive probes containing a base sequence complementary to the RFLP’s being identified

  • The nylon sheet is then placed against X-ray film and is exposed for several days

    • Bands appear where radioactive probes stuck to fragments on the nylon sheet

    • Usually 2 bands will show up (one RFLP from each chromosome)

      • Bands are analyzed to see similarities and/or differences


PCR

  • Is a technique used for making copies of a defined segment of a DNA molecule

  • Can be valuable when the amount of evidence is minimal

    • Millions of copies of DNA can be made from a single speck of blood

      • DNA taken from white blood cells, not red blood cells


PCR

  • Steps involved

    • Heat the DNA strands

      • Causes the strands to separate

    • Cool the mixture and add a primer

      • Is a short sequence of base pairs that will add to its complementary sequences on the DNA strand

    • Add DNA polymerase and a mixture of free nucleotides to the separated strands

    • Heat again to around 75 degrees Celsius for the completion


PCR

  • The outcome of one cycle of PCR is a doubling of the number of DNA strands

  • Usually 25-30 cycles of PCR occur

    • Yield more than 1 million copies of the original DNA molecule

  • Each cycle takes 2 minutes to complete


PCR

  • Advantages

    • Minute amounts of DNA may be used for amplification

    • DNA degraded to fragments only a few hundred base pairs in length can serve as effective templates for amplification

    • Large numbers of copies of specific DNA sequences can be amplified simultaneously


PCR

  • Disadvantages

    • Contaminant DNA, such as those contaminated with fungus or bacteria, will not amplify


Electrophoresis
Electrophoresis

  • Is a technique used to separate DNA fragments

  • An electrical current is moved through a gel substance causing molecules to sort by size

  • The smaller, lighter molecules will move the furthest on the gel

  • After developing, the fragments can be visualized for characterization


Electrophoresis1
Electrophoresis

  • Steps

    • Pipette the DNA

    • Load DNA into the gel wells

    • Run the gel

    • Observe and compare bands of DNA


Short tandem repeats
Short Tandem Repeats

  • AKA STR

  • Are locations on the chromosome that contain short sequences of 2-5 bases that repeat themselves in the DNA molecule

    • Entire strand of DNA is less than 450 bases in length

  • Is the most used procedure for DNA typing

  • Advantages

    • Provides greater discrimination

    • Requires less time

    • Requires smaller sample size

    • DNA is less susceptible to degradation


Short tandem repeats1
Short Tandem Repeats

  • Procedure

    • Extract the gene TH01 from the sample

      • TH01 has seven human variants with a repeating sequence of AATG

    • Amplify the sample by means of PCR

    • Separate by electrophoresis

    • Examine the distance the STR migrates to determine the number of times TH01 repeats


Short tandem repeats2
Short Tandem Repeats

  • Each person has two STR types for TH01

    • One inherited from each parent

  • By continuing the process with additional STR’s from other genes, you can narrow down the probability of DNA belonging to only one person


Short tandem repeats3
Short Tandem Repeats

  • STR typing is visualized by peaks shown on a graph

    • Each represents the size of the DNA fragment

  • The possible alleles are numbered for each location (loci)


Short tandem repeats4
Short Tandem Repeats

  • http://www.dnai.org/d/index.html


Y str
Y-STR

  • Is another tool available to type STR’s located on the Y chromosome

    • Is male specific

  • More than 20 different Y-STR markers have been identified

  • Useful when multiple males are involved in a sexual assault

  • Analysis will have only one band or peak

  • Is less complicated in appearance and interpretation


Outcomes to dna typing
Outcomes to DNA Typing

  • 3 outcomes

    • Match

      • DNA profile appears the same

      • Lab determines the frequency

    • Exclusion

      • The genotype comparison shows profile differences that can only be explained by the two samples originating from different sources

    • Inconclusive

      • The data does not support a conclusion as to whether the profiles match


Types of dna

Nuclear

Found in the nucleus

Constitutes 23 pairs of chromosomes inherited from both parents

Each cell contains only one nuclei

Mitochondrial

Found in the cytoplasm

Is inherited only from the mother

Each cell contains hundreds to thousands of mitochondria

Can be found in skeletal remains

Types of DNA


Mitochondrial dna
Mitochondrial DNA

  • Analysis of mDNA is more

    • Rigorous

    • Time consuming

    • Costly than nucleic testing of DNA

  • mDNA is constructed in a circle or loop

  • 37 genes are involved in mitochondrial energy generation

  • Is used when nuclear DNA typing is not possible


Codis
CODIS

  • Stands for Combined DNA Index System

  • Used for linking serial crimes and unresolved cases with repeat offenders

  • Launched October 1998

  • Links all 50 states

    • Locally, statewide, nationally

  • Requires >4 RFLP markers and/or 13 core STR markers


Packaging biological evidence
Packaging Biological Evidence

  • Before packaging, photograph and record evidence on sketches

  • Wearing disposable latex gloves while handling the evidence is required

  • Clothing from victim and suspect with blood evidence must be collected

  • The packaging of biological evidence in plastic or airtight containers must be avoided

    • Moisture can contribute to growth of DNA-destroying bacteria and fungi


Packaging biological evidence1
Packaging Biological Evidence

  • Each stained article should be packaged separately in a paper bag or in a well- ventilated box

  • Dried blood is best removed using a sterile cotton swab lightly moistened with distilled water

    • Is air dried before being placed in a swab box, then a paper or manilla envelope

  • All biological evidence should be refrigerated or stored in a cool location until delivery to the lab

  • Standard/reference DNA specimens must also be collected


ad