Figure 1a e supplement
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Figure 1a-e, supplement PowerPoint PPT Presentation


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e. a. b. unstimulated. +fMLP. T308P-Akt. S473P-Akt. Akt. c. d. (min). 0 0.5 1 1.5 2 4. + fMLP (100 nM). PH-Akt-GFP. Anti-PIP 3 antibody. Figure 1a-e, supplement. f. (min). 0. 1. 1.5. 0.5. 2. 4. + fMLP (100 nM). Figure 1f, supplement. a. Overlay. DiD.

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Figure 1a-e, supplement

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Figure 1a e supplement

e

a

b

unstimulated

+fMLP

T308P-Akt

S473P-Akt

Akt

c

d

(min)

0 0.5 1 1.5 2 4

+ fMLP (100 nM)

PH-Akt-GFP

Anti-PIP3 antibody

Figure 1a-e,

supplement


Figure 1a e supplement

f

(min)

0

1

1.5

0.5

2

4

+ fMLP (100 nM)

Figure 1f,

supplement


Figure 1a e supplement

a

Overlay

DiD

PH-Akt-GFP

b

Ratio

(PH-Akt-GFP/DiD)

Fig. 2a-b, supplement


Figure 1a e supplement

c

Fig. 2c, supplement


Figure 1a e supplement

a

HL-60

+ 50 nM wort

Figure 3a, supp


Figure 1a e supplement

d

b

120

100

80

Relative level of

T308P-Akt (%)

60

+ 200 nM wort

40

c

20

0

120

T308P-Akt

100

80

S473P-Akt

Relative level of

S473P-Akt (%)

60

40

Akt

20

0

1

2

3

4

5

6

7

HL-60

+ 50 mM LY

+ 200 mM LY

+ 400 mM LY

HL-60

+ 20 nM wort

+ 50 nM wort

HL-60 +fMLP

+ 200 nM wort

+ 50 mM LY

+ 200 mM LY

+ 400 mM LY

+ 20 nM wort

+ 50 nM wort

+ 200 nM wort

Figure 3b-d, supplement


Figure 1a e supplement

a

HL-60

+ wort

Paths

10 min

6 min

2 min

c

b

+ fMLP

150

HL-60

100

50

3

0

2

4

1

HL-60

HL-60

+ wort

+ 50 nM wort

+ 200 nM wort

Figure 4, supplement


Figure 1a e supplement

b

a

4000

Cell Number

2000

0

0

0.1

1

10

100

Human blood neutrophils

Log [fMLP] (nM)

c

+ 30 nM wortmannin

PLB-985

+ 200 mM LY 294002

Figure 5, supplement


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