Measuring fat and vacuolar space with digital pathology
Download
1 / 32

Measuring Fat and Vacuolar Space with Digital Pathology - PowerPoint PPT Presentation


  • 69 Views
  • Uploaded on

Measuring Fat and Vacuolar Space with Digital Pathology. Frank Voelker, DVM, DACVP Principle Pathologist. Trevor Johnson, MS Director, Image Analysis. Originally An Algorithm for Measuring Fat. What was actually needed: An algorithm for measuring circumscribed clear spaces in tissue.

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about ' Measuring Fat and Vacuolar Space with Digital Pathology' - larya


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
Measuring fat and vacuolar space with digital pathology

Measuring Fat and Vacuolar Space with Digital Pathology

Frank Voelker, DVM, DACVP

Principle Pathologist

Trevor Johnson, MS

Director, Image Analysis


Originally an algorithm for measuring fat
Originally An Algorithm for Measuring Fat

What was actually needed: An algorithm for measuring circumscribed clear spaces in tissue

  • White Fat

  • Brown Fat

  • Lipid vacuoles in a variety of tissues (eg. Fatty liver or adrenal cortex)

  • Tissues composed of fibers or tubules (eg. skeletal muscle)

  • Tissues with hollow spaces or glandular lumena (eg. lung or thyroid gland)

  • Cerebral edema, demyelinization

  • Vacuoles in various cell types (eg. Lymphocytes)


Algorithm requirements
Algorithm Requirements

  • Data Output:

  • Percent area of vacuoles or circumscribed spaces.

  • Total number of spaces

  • Mean area/diameter/perimeter of spaces

  • Histograms of areas, diameters or perimeters

  • Shape and Area Threshold Controls

  • Hue Sensitivity Adjustment

  • Membrane Estimation or Completion


Vacuole algorithm settings
Vacuole Algorithm Settings

Input and Output Windows


How the image is analyzed
How the Image is Analyzed

Define the tissue intensity

Determine the maximum and minimum vacuole areas

Determine how close each vacuole is allowed to resemble a circle and how tightly they are packed

Determine if Vacuole Perimeter Estimation (VPM) is needed












Fatty liver
Fatty Liver Vacuoles




Measuring brown fat with nuclear counting
Measuring Brown Fat with Nuclear Counting Vacuoles

173 Nuclei

Area/No. Nuclei = Cell Size

No. Nuclei/No. Fat Droplets = No. Droplets/Cell




Adrenal gland
Adrenal Gland Vacuoles







Measuring skeletal muscle fibers
Measuring Skeletal Muscle Fibers Vacuoles

Type 1- Red Muscle. Slow twitch postural type muscle fiber. Aerobic oxidation.

Type 2- White Muscle Fast twitch fibers. ATPase +. Anaerobic oxidation

Muscle fiber alterations are important in:

Denervation

Statin-induced rhabdomyolysis

Noninflammatory myopathies

Congential myopathies

Sports physiology


Measuring skeletal muscle fibers1
Measuring Skeletal Muscle Fibers Vacuoles

In H&E sections, skeletal muscle fibers are poorly demarcated




Summary
Summary Vacuoles

An algorithm designed to measure vacuoles or circumscribed clear spaces in tissue was described in this presentation. The algorithm has proved useful in measuring clear spaces in a variety of tissues including white and brown fat, skeletal muscle fibers, pulmonary alveoli, fatty liver and adrenal cortex. Data outputs included percent as well as histograms and mean areas, diameters or perimeters of vacuoles or clear areas within tissue.


ad