Avena genetic resources for quality in human consumption (AVEQ)
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Avena genetic resources for quality in human consumption (AVEQ). Partner 1 2 IHAR -PIB Laboratory of Quality Evaluation of Plant Materials Institute of Plant Breeding and Acclimatization - National Research Institute , Radzików, PL 05 - 870, Poland.

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Avena genetic resources for quality in human consumption (AVEQ)

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Avena genetic resources for quality in human consumption (AVEQ)

Partner 12 IHAR -PIB

Laboratory of Quality Evaluation of Plant Materials

Institute of Plant Breeding and Acclimatization - National Research Institute,

Radzików, PL 05-870, Poland

AVEQ Final Meeting – BucharestOctober 19-21, 2010


WP 7. Task 1, Sub-task 4 .

Analyses for starch and dietary fibre

The objective: Characterise carbohydrates - starch and dietary fibre in genetic resources of Avena.

AVEQ Final Meeting – Bucharest October 19-21, 2010


WP 7. Task 1, Sub-task 4 .

Analysis of starch - selected method

-amylase/amyloglucosidase method:

ICC Standard no. 128/1and168

or AACC 76-13

or AOAC 996.11

AVEQ Final Meeting – Bucharest October 19-21, 2010


WP 7. Task 1, Sub-task 4 .

Analysis of dietary fibre - selected method

Enzymatic-Chemical Uppsala Method (AOAC 994.13 or AACC 32-25)

Gas chromatographic-colorimetric-gravimetric procedure

  • TDF = neutral sugar residues + uronic acids + Klason lignin

  • Principle

  • 1. Measurement of NSP as neutral sugar residues by GLC

  • (Englyst and Cummings procedure, 1988)

  • starch removal by specific enzymes

  • precipitation of soluble polymers by ethanol

  • hydrolysis of polysaccharides to monosaccharides with H2SO4

  • reduction and derivatisation

  • 2. Measurement of uronic acid by colorimetry (Scottmethod, 1979)

  • 3. Measurement of Klason lignin by gravimetric method as the sulphuric acid resistant residue (Theander et al.,1995)


Dietary Fibre Constituents

I. Non-starch polysaccharides & oligosaccharides

Cellulose

Non-cellulosic polysaccharides

  • Polyfructoses

  • inulin

  • oligofructans

  • Galactooligosugars

  • Hemicelluloses

  • arabinoxylans

  • arabinogalactans

  • β-glucans

  • Pectins

  • Gums

  • Mucilages

IV. Substances associated with NSP & lignin complex in plants

II. Analogous Carbohydrates

Waxes

Phytates

Cutin

Suberin Saponins

Tannins

Resistant starch

Synthesized carbohydr.

Indigestible dextrins

III. Lignins

AVEQ Final Meeting – Bucharest October 19-21, 2010


WP 7. Task 1, Sub-task 4 .

Analyses for starch and dietary fibre

Material analysed from the year 2008

- 60 samples of selected accessions from trial in Bulgaria

- 61 samples of selected accessions from trial in Estonia

- 2 samples of wild accessions from trial in Estonia

- 6 samples of wild accessions from trial in Poland

- 53 samples of standards from trial in Estonia

Total no of accessions: 182

AVEQ Final Meeting – Bucharest October 19-21, 2010


WP 7. Task 1, Sub-task 4 .

Analyses for starch and dietary fibre

I set - selected 60 accessions from Bulgaria trial

Range 51.9 – 64.1%; avg – 56.3%; CV – 4.3%


WP 7. Task 1, Sub-task 4 .

Analyses for starch and dietary fibre

I set - selected 60 accessions from Bulgaria trial

TDF - range 9.4–18.2% avg - 12.0%; CV - 10%

5.0

2.6

6.1

14.1

NSP - range 6.1–14.1%

avg - 8.8 %; CV - 13%

Uronic acids - range 0.14–0.32%

avg – 0.23%; CV - 18%

Lignin - range 2.6–5.0%

avg - 3.3%; CV - 13%


WP 7. Task 1, Sub-task 4 .

Analyses for starch and dietary fibre

II set - selected 61 accessions from Estonia trial

Range 48.9 – 64.9%; avg – 58.7%; CV – 5.9%


WP 7. Task 1, Sub-task 4 .

Analyses for starch and dietary fibre

II set - selected 61 accessions from Estonia trial

TDF - range 9.0–15.8% avg - 11.2%; CV - 12%

4.4

1.8

11.8

6.5

NSP - range 6.5–11.8%

avg - 8.2 %; CV - 13%

Uronic acids - range 0.12–0.24%

avg – 0.17%; CV - 16%

Lignin - range 1.8–4.4%

avg - 2.8%; CV - 22%


WP 7. Task 1, Sub-task 4 .

Analyses for starch and dietary fibre

II set - selected 61 accessions from Estonia trial plus 2 wild Avena samples

TDF - range 9.0–15.8% avg - 11.2%; CV - 12%

NSP - range 6.5–11.8%

avg - 8.2 %; CV - 13%

Uronic acids - range 0.12–0.24%

avg – 0.17%; CV - 16%

Lignin - range 1.8–4.4%

avg - 2.8%; CV - 22%


WP 7. Task 1, Sub-task 4 .

Analyses for starch and dietary fibre

Polish wild species

AVEQ Final Meeting – Bucharest October 19-21, 2010


WP 7. Task 1, Sub-task 4 .

Analyses for starch and dietary fibre - standards


WP 7. Task 1, Sub-task 4 .

Relationship between NSP on TDF content

TDF

[% DM]

NSP [% DM]

AVEQ Final Meeting – Bucharest October 19-21, 2010


WP 7. Task 1, Sub-task 4 .

Analyses for starch and dietary fibre

  • Plans for the next four months:

  • Additional standard samples (53 samples of 11 cultivars from field experiment in Estonia) at present are analyzing for dietaryfibreand they shouldbe completed within 10 day from now.

  • Analyses for starch and TDF (NSP only) in 70 selected accessions from trial inPoland (2008) and France, Sweden and Italy (2009).

AVEQ Final Meeting – Bucharest October 19-21, 2010


WP 7. Task 1, Sub-task 4 .

Analyses for starch and dietary fibre

  • This work has been carried out by :

  • Anna Fraś

  • Olga Jablonka

  • Danuta Boros

  • With appreciable help of two technicians:

  • Magdalena Bodzon

  • Monika Skonieczna

AVEQ Final Meeting – Bucharest October 19-21, 2010


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