1 / 4

Round2

Date: 27 May 2010 Exp: RNA game 4. PCR result; 1 pot type: construct No. 2 get non specific product 2 Round type; all works!. 1 2 3 4 5 6 7 8. 1 2 3 4 5 6 7 8. Transcription result; 1 pot type & 2 Round type; all works , in different amount of RNA. Round2.

lajos
Download Presentation

Round2

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Date: 27 May 2010 Exp: RNA game 4 • PCR result; • 1 pot type: construct No. 2 get non specific product • 2 Round type; all works! 1 2 3 4 5 6 7 8 1 2 3 4 5 6 7 8 Transcription result; 1 pot type & 2 Round type; all works, in different amount of RNA Round2 ----- 0 Hrs ----- ------ 3 Hrs ---- 1 2 3 4 5 6 7 8 1 2 3 4 5 6 7 8

  2. I show you sequencing data by using RNA made from R2, construct 1 - 8 No.1 = 2782 ( Stephanie’s 3rd) No.2 = 2785 (Hui’s Cross 2) No.3 = 2788 (Chris’s B Cross2) No.4 = 2791 (Jee’sBulgeless Cross 1) No.5 = 2792 (Jee’sBulgeless Cross 2) No.6 = 2793 (Jee’s Cross 3) No.7 = 2794 (Adrien’sBulgeless1) No.8 = 2795 ((Adrien’sBulgeless2) Abbreviations: NM Abbreviations; NM = No modify ddGTP = use dideoxy-Guanine nucleotide asa internal ladder SH = SHAPE reaction As NM as a background Construct No.3 = 2788 (Chris’s B Cross2) and No.6 = 2793 (Jee’s Cross 3) seem to be a cleanest construct. Please look at the SHAPE data, last column of each construct result; Construct 2 – 7 show the region that corresponds to ‘the loop” [Red arrow], please see the structure image.

  3. Next thing to do; 1) From the latest result, Chris can assign the sequence from this data. 2) Somehow, my result on ddNTPs should be repeat. Once I finish, I will pass the result to Chris ASAP. See you all at the meeting! Regards, Ann

More Related