Acinetobacter 89
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2. 1. Группа Acinetobacter (89%) 93% symbiont Alvinella pompejana (Campbell et.al., unpublished). Рис.1 Результаты DGGE Камчатских проб с Bact. и Arch. праймерами. Форез 1 : NPT1 Bact. pr. и 47K Bact. pr. ( градиент 35-65% ) Форез 2 : NPT1 Bact. pr. (градиент 35-55%)

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Группа Acinetobacter (89%)

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Acinetobacter 89

2

1

Группа Acinetobacter (89%)

93% symbiont Alvinella pompejana (Campbell et.al., unpublished)

Рис.1 Результаты DGGE Камчатских проб с Bact. и Arch. праймерами.

Форез 1: NPT1 Bact. pr. и 47K Bact. pr. (градиент 35-65%)

Форез 2: NPT1 Bact. pr. (градиент 35-55%)

Форез 3: NPT1 Bact. и Arch pr.; 47K Bact. pr. (градиент 35-65%)

NPT1

3

Группа Pseudomonas, Acinetobacter (родственников термофилов не обнаружено)

NPT1

47K

NPT1

NPT1

47K

96% Uncultured bact. clones Napoli and Urania (Heijs et.al.)

95% Uncultured bact. clone (Zhao et.al., unpublished, deepsea sediment from the Pacific Warm Pool)

95% Uncultured bact. (Madrid et.al., 2001, anoxic zone of the Cariaco Basin)

Группа Acinetobacter (87%)

87% Uncultured gamma-Proteobact. (Bonheyo et.al. unpublished, microbial diversity, Yellowstone Park)

Группа Brevundimonas (97% )

97% Uncultured clones Urania (Heijs et.al.)

96% Uncultured bact. clones Napoli and Urania (Heijs et.al.)

93% Uncultured bact. (Laniol et.al.., 2001, Bact. and Arch. associated with Gulf of Mexico gas hydrates)

Группа Acinetobacter (93%)

91% symbiont Alvinella (Campbell et.al.)

93% Thermophic anaerobic bact. (Huang et.al., unpublished, Guguan hot spring)

93% Uncultured bact. (Godon et.al., unpublished, anaerobic digestor)

Группа Rhizobiales (Ochrobactrum (88%), Brucella (87%)

(Термофилы не обнаружены))

Группа Cytophaga/Bacteroidetes (92%)

Группа Acinetobacter

91% symbiont Alvinella

96% Uncultured bact. clones Napoli and Urania (Heijs et.al., unpublished, deepsea mud volcanoes in eastern Mideterranean)

93% Uncultured bact. clone (Zhao et.al., unpublished, deepsea Western Pacific )

1 Группа Thermoanaerobacter (93%)

93% delta-Proteobact. (Alain et.al., 2003, bacterial assemblage associated with polychaete of the hydrothermal vent)

91% Thiobacillus thermosulfates (небольшой участок сходства)

96% Uncultured bact. Clones Napoli and Urania

97% Uncultured bact. (Nakagawa et.al. Japanese hot spring)

96% Dictyoglomus thermophilum

95% Uncult.bact. clone (Spear et.al., 2005, Yellowstone Park)

Группа Acinetobacter (97%)

89% Uncultured bact. 01463 (Orphan et.al., 2000, high temperature oil reservoirs)

Группа Acinetobacter (89%)

89% Uncultured bact. 01463 (Orphan et.al., 2000, high temperature oil reservoirs)

82% Uncultured bact. (Donachic et.al., unpublished, microbial diversity in the Hawaiian Archipelago)

82% Uncultured bact.(Orphan et.al., 2000, petroleum reservoirs)

Группа Brevundimonas = Pseudomonas (α-Proteobact. 97%)

97% Uncultured clone Urania (Heijs et.al.)

97% Uncultured bact. (Inagaki et.al.,2003, Japanese epitharmal gold mine)

Группа Brevundimonas (α-Proteobact. 95%)

97% Uncultured clone Urania (Heijs et.al.)

97% Uncultured bact. (Inagaki et.al.,2003, Japanese epitharmal gold mine)


Acinetobacter 89

2

1

Группа Acinetobacter (89%)

93% symbiont Alvinella pompejana (Campbell et.al., unpublished)

Рис.1 Результаты DGGE Камчатских проб с Bact. и Arch. праймерами.

Форез 1: NPT1 Bact. pr. и 47K Bact. pr. (градиент 35-65%)

Форез 2: NPT1 Bact. pr. (градиент 35-55%)

Форез 3: NPT1 Bact. и Arch pr.; 47K Bact. pr. (градиент 35-65%)

NPT1

3

Группа Pseudomonas, Acinetobacter (родственников термофилов не обнаружено)

NPT1

47K

NPT1

NPT1

47K

96% Uncultured bact. clones Napoli and Urania (Heijs et.al.)

95% Uncultured bact. clone (Zhao et.al., unpublished, deepsea sediment from the Pacific Warm Pool)

95% Uncultured bact. (Madrid et.al., 2001, anoxic zone of the Cariaco Basin)

Группа Acinetobacter (87%)

87% Uncultured gamma-Proteobact. (Bonheyo et.al. unpublished, microbial diversity, Yellowstone Park)

Группа Brevundimonas (97% )

97% Uncultured clones Urania (Heijs et.al.)

96% Uncultured bact. clones Napoli and Urania (Heijs et.al.)

93% Uncultured bact. (Laniol et.al.., 2001, Bact. and Arch. associated with Gulf of Mexico gas hydrates)

Подобные клоны обнаружены в воде

Группа Acinetobacter (93%)

91% symbiont Alvinella (Campbell et.al.)

93% Thermophic anaerobic bact. (Huang et.al., unpublished, Guguan hot spring)

93% Uncultured bact. (Godon et.al., unpublished, anaerobic digestor)

Группа Rhizobiales (Ochrobactrum (88%), Brucella (87%)

(Термофилы не обнаружены)

Группа Cytophaga/Bacteroidetes (92%)

Группа Acinetobacter

91% symbiont Alvinella

96% Uncultured bact. clones Napoli and Urania (Heijs et.al., unpublished, deepsea mud volcanoes in eastern Mideterranean)

93% Uncultured bact. clone (Zhao et.al., unpublished, deepsea Western Pacific )

1 Группа Thermoanaerobacter (93%)

93% delta-Proteobact. (Alain et.al., 2003, bacterial assemblage associated with polychaete of the hydrothermal vent)

91% Thiobacillus thermosulfates (небольшой участок сходства)

96% Uncultured bact. Clones Napoli and Urania

97% Uncultured bact. (Nakagawa et.al. Japanese hot spring)

96% Dictyoglomus thermophilum

95% Uncult.bact. clone (Spear et.al., 2005, Yellowstone Park)

Группа Acinetobacter (97%)

89% Uncultured bact. 01463 (Orphan et.al., 2000, high temperature oil reservoirs)

Группа Acinetobacter (89%)

89% Uncultured bact. 01463 (Orphan et.al., 2000, high temperature oil reservoirs)

82% Uncultured bact. (Donachie et.al., unpublished, microbial diversity in the Hawaiian Archipelago)

Подобные клоны обнаружены в воде

82% Uncultured bact.(Orphan et.al., 2000, petroleum reservoirs)

Группа Brevundimonas = Pseudomonas (α-Proteobact. 97%)

97% Uncultured clone Urania (Heijs et.al.)

97% Uncultured bact. (Inagaki et.al.,2003, Japanese epitharmal gold mine)

Группа Brevundimonas (α-Proteobact. 95%)

97% Uncultured clone Urania (Heijs et.al.)

97% Uncultured bact. (Inagaki et.al.,2003, Japanese epitharmal gold mine)


Acinetobacter 89

Плохой сиквенс (Mus. ьusculus)

96% Carboxydobrachium pacificus

96% Thermoanaerobacter tengcongensis

NPT1 019 019 1321 1321

92% Carboxydocella thermoautotrophica

93% Carboxydocella sporoforma

47K NPT1 NPT1 019 019 1321 1321

H2O

95% Uncultured bact. (Nakagawa et.al., 2002, thermophilic sulfate-reducing bact. Within Cu-Pb-Zn mine)

94% Carboxydocella sporoforma

93% Carboxydocella thermoautotrophica

Группа Сytophaga/ Flexibacter/Bacteroidetes

95% Thermoanaerobacter tengcongensis

94% Carboxydobrachium pacificus

Группа Acinetobacter (Почти все некульт.)

95% Uncultured bact. (Elsaie et.al., 2004, deep-sea hydrothermal vent water, Japan)

95% Uncultured bact. (Takami et.al., 1997, deepest sea mud)

96% Carboxydocella thermoautotrophica

96% Carboxydobrachium pacificus

96% Thermoanaerobacter tengcongensis

Сиквенс не получился

1

96% Carboxydobrachium pacificus

96% Thermoanaerobacter tengcongensis

2

97% Uncultured bact. clones Napoli and Urania (Heijs et.al., unpublished, deepsea mud volcanoes in eastern Mideterranean)

97% Uncultured bact. (Takami et.al., 1997)

97% Uncultured bact. (Inagaki,Horikoshi et.al., 2003, Japan epithermal gold mine )

93% Carboxydocella thermoautotrophica

3

95% Carboxydocella thermoautotrophica

96% Carboxydocella sporoforma

96% Группа Desulfotomaculum и Thermoanaerobacter

95% Tab. tengcongensis

96% Carboxydobrachium pacificus

96% Thermoanaerobacter tengcongensis

Группа Thermoanaerobacter

95% Tab. siderofilus

Группа -Proteobact. Низкое сходство

89% Uncultured bact. (Miyoshi et.al., unpublished, deep sea hydrothermal vent)

Рис. 2 Результаты DGGE Камчатских проб и накопительных культур с Bact. Праймерами (денатурирующий градиент 35%-65%). Дорожки: 1-NPT1, 2-019/H2+Fe, 3-019, 4-1321/H2, 5-1321, 6-H2O, 7-47K, 8-NPT1, 9-NPT1, 10-019/H2+Fe, 11-019, 12-1321/H2, 13-1321.

H2O: 1-Сиквенс не получен, 2-группа Pseudomonas

89% Bact. (Donachie et.al., unpublished, microbial diversity in the Hawaiian Archipelago)

89% Uncultured bact. (Lanoil et.al., 2001, Bact. and Arch. associated with Gulf of Mexico gas hydrates),

3-группа Pseudomonas

93% Uncultured gamma-Proteobact. (Li et.al., 1999, deepest cold-seep area)

93% Uncultured bact. (Donachie et.al., unpublished)

95% Carboxydobrachium pacificus

94% Thermoanaerobacter tengcongensis


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