Variability of antarctic picoeukaryotic assemblages studied by two fingerprintins methods
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Variability of Antarctic picoeukaryotic assemblages studied by two fingerprintins methods. Beatriz Díez 1 , Terence L. Marsh 2 , Ramon Massana 1 and Carlos Pedrós-Alió 1

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Variability of Antarctic picoeukaryotic assemblages studied by two fingerprintins methods

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Variability of antarctic picoeukaryotic assemblages studied by two fingerprintins methods

Variability of Antarctic picoeukaryotic assemblages studied by two fingerprintins methods

Beatriz Díez1, Terence L. Marsh2 , Ramon Massana1 and Carlos Pedrós-Alió1

1Departament de Biologia Marina, Institut de Ciències del Mar, CSIC, Passeig Joan de Borbó s/n, E-08039 Barcelona, Catalunya, Spain

2Center of Microbial Ecology and Department of Microbiology, 41 Gilner Hall, Michigan State University, East Lansing, 48824 Michigan, USA


Variability of antarctic picoeukaryotic assemblages studied by two fingerprintins methods

Southern Ocean: area studied


Variability of antarctic picoeukaryotic assemblages studied by two fingerprintins methods

DHARMA cruise

DOVETAIL cruise


Variability of antarctic picoeukaryotic assemblages studied by two fingerprintins methods

Objectives

Study the distribution and variability of eukaryotic picoplankton in Southern Ocean

Comparison of DGGE and T-RFLP results

Identification of the dominant populations by both techniques

Emphasis on the distribution of novel phylogenetic groups: stramenopiles and alveolates


Variability of antarctic picoeukaryotic assemblages studied by two fingerprintins methods

ICE-EDGE

DOVETAIL transect

DHARMA transect

EDGE-ICE


Variability of antarctic picoeukaryotic assemblages studied by two fingerprintins methods

DOVETAIL Surface

T-RFLP (MspI)

Mantoniella

DOV1

DOV2

DOV3

DOV4

Phaeocystis

DOV5

DOV6


Variability of antarctic picoeukaryotic assemblages studied by two fingerprintins methods

DOVETAIL transect

DGGE

T-RFLP (MspI and RsaI)

ICE-EDGE

ICE

-EDGE

A=SURFACE

B=Bottom of mixed layer


Variability of antarctic picoeukaryotic assemblages studied by two fingerprintins methods

19**

18

17**

15

16

14

10

13

9

12

11

8

7*

6

5

4

3

2

1


Variability of antarctic picoeukaryotic assemblages studied by two fingerprintins methods

Edge-ice

SAF

DH32

DH12

DH18

DH24

DH1

Dharma 5 - 0.2µm

DH1= Ice-edge

DH12= Scotia Front

DH24= Polar Front

DH32= Subantartic Front


Variability of antarctic picoeukaryotic assemblages studied by two fingerprintins methods

DHARMA

DGGE

T-RFLP (MSPI)

ICE

ICE

WSF

ICE

WSF

ACC

ACC

WSF

PF

PF AND SAF

SAF


Variability of antarctic picoeukaryotic assemblages studied by two fingerprintins methods

DHARMA CYTOMETRY

DH11 ---

+-----

DH14 --- |

+-----

DH18 -- | |

+-- | |

DH12 -- | | |

+---- |

DH5 - | |

+- | |

DH9 -| | |

+-- |

DH20 -- |

+-------------------------------

DH22 -- |

+-----------

DH3 -+

|

DH1 --

DH24 ------

+--------------------------------------

DH28 - |

| |

DH26 + |

| |

DH32 + |

+-----

DH30 -

PF


Variability of antarctic picoeukaryotic assemblages studied by two fingerprintins methods

*Novel alveolates

**Novel stramenopiles


Variability of antarctic picoeukaryotic assemblages studied by two fingerprintins methods

DH18 (WSF)

19**

18

17**

16

10

13

12

11

8

7*

6

5

4*

10 25 60 100 250 500 1000 2000 3000

Depth (meters)


Variability of antarctic picoeukaryotic assemblages studied by two fingerprintins methods

Conclusions

DGGE and T-RFLP fingerprinting approaches provide information about changes in population distribution. Also they offer the possibility to identify the dominant components of the natural populations.

When the same size fractions were compared, the assemblages were rather similar in the two transects.

Despite the high resolution of the T-RFLP technique, usually more than one species had the same restriction site in the 18S rDNA, resulting in an identical peak size. The most likely candidate must be decided with information from other techniques


Variability of antarctic picoeukaryotic assemblages studied by two fingerprintins methods

Although diatoms have been usually found to be dominant in Antarctic algal blooms and are the best known group, it is clear that small-celled species (<5 µm) and not only autotrophic organisms, are very important in many antarctic locations

Similar phylogenetic groups and dominant species were detected by DGGE and T-RFLP.

Prasinophyceae, Prymnesiophyceae, Bacillariophyceae, Novel stramenopiles, Dinophyceae, and Novel alveolates, were found to be the most widespread groups, further supporting the suggestion that these organisms are common and widely distributed both in Antarctic waters and in most Oceans


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