Wu kuan yi
This presentation is the property of its rightful owner.
Sponsored Links
1 / 9

Wu, Kuan-Yi PowerPoint PPT Presentation


  • 99 Views
  • Uploaded on
  • Presentation posted in: General

Wu, Kuan-Yi. Properties of minD/ ParA superfamily. Structurally similar ATPase activity Polymerization Other playes dynamic oscillation. (ParA). YFP-MinD/ MinE-CFP. The relationship between players. Placement of division sites C: inhibitor of septation D: membrane recruitment of C

Download Presentation

Wu, Kuan-Yi

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -

Presentation Transcript


Wu kuan yi

Wu, Kuan-Yi


Properties of mind para superfamily

Properties of minD/ ParA superfamily

  • Structurally similar

  • ATPase activity

  • Polymerization

  • Other playes

  • dynamic oscillation

(ParA)


The relationship between players

YFP-MinD/ MinE-CFP

The relationship between players

Placement of division sites

  • C: inhibitor of septation

  • D: membrane recruitment of C

  • E: topological specificity

    DNA partition

  • A: recruited by B

  • B: recognize parS

  • S: cis-element in arrays


Wu kuan yi

MinC+MinD

C MinC

DMinD

E MinE

Division inhibition

MinC concentration

Zone of division inhibition

MinE

Septal FtsZ ring

MinCDE polar zone

pole-to-pole

oscillation

Placement of Z-ring


Wu kuan yi

1

ADP

MinD

MinD

- - -

+ + +

5

P

MinC

MinE

2

MinE

3

ATP

ATP

ATP

ATP

MinC

MinE

4

MinC

ATP

ATP

MinD

MinD

MinD

MinD

MinD

Molecular mechanism of the oscillating Min system

C-terminus


Wu kuan yi

In vitro

In vivo

MinD

MinE

ATP

Phospholipid vesicle

ParA

ParB

ATP


Localization of para1 scoe in different e coli strains

A22

Localization of parA1scoe in different E.coli strains

A22 treatment

ΔmreB


Wu kuan yi

MC1000/ pSOT1013

Live cell image

YFP

DIC

merge

DAPI

Oscillate with minD?  minD binding site

Move independently membrane targeting?

Existence of another proteins

In the presence or absence of parB


Test on ori ter localization

Test on ori/ ter localization

YFP

DIC-YFP

DIC

CFP

IL2/ pLAU53

YFP

DIC-CFP

DIC

CFP

IL29/ pLAU53


  • Login