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Single particle processing workflow in Bsoft

Single particle processing workflow in Bsoft. Bernard Heymann. SPA Processing Steps. Picking Corrections/Modifications (CTF, mask) Alignment Reconstruction Quality assessment (Resolution). Bsoft SPA Workflow. Parameter Organization. Parameter Handling. Flat files:

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Single particle processing workflow in Bsoft

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  1. Single particle processing workflow in Bsoft • Bernard Heymann

  2. SPA Processing Steps • Picking • Corrections/Modifications (CTF, mask) • Alignment • Reconstruction • Quality assessment (Resolution)

  3. Bsoft SPA Workflow

  4. Parameter Organization

  5. Parameter Handling • Flat files: • Text files with tagged parameters • Images referenced through tags • Multiple formats: STAR & XML • Backwards compatible • Types of Bsoft parameter files: • Micrograph/reconstruction parameter files • Modeling parameter files

  6. Quality Assessment • Reconstruction: • Resolution/spatial frequency limit. • Modification curve (sharpening, B-factor) • Number of particles in reconstruction • Defocus range, astigmatism range • View distribution • Coverage/Completeness (up to spatial frequency limit) • Particle FOM distribution • Resolution: FSC curve or similar • Modeling • Fitting statistics

  7. Reconstruction assessment View distribution FOM distribution Resolution

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