بسم الله الرحمن الرحيم. Conjuctival Discharge. 2013-2014. Diagnostic Medical Microbiology-Laboratory Manual. Conjunctival Discharge. Aim of the test
Diagnostic Medical Microbiology-Laboratory Manual
Aim of the test
An etiological diagnosis of bacterial conjunctivitis by aerobic cultivation with identification and susceptibility test of the isolated bacteria.
Types of specimen
Two swabs from discharge from the eye(s).
Criteria of specimen rejection
Inappropriate specimen transport device; mislabeled specimen; unlabeled specimen; specimen received after prolonged delay (usually more than two hour); specimen received in expired transport media.
Who is authorized to order the test
Quantity of specimen
Sufficient amount on swab.
Time relapse before processing the sample
Eye specimen should be processed immediately because tears contains lysosomes which may kill the organism.
Maintain specimen swab at room temperature .Don’t refrigerate.
Conjunctival discharge Specimen collection:-
Pull down the lower eyelid so that the lower conjunctival fornix is exposed.
Swab the fornix without touching the rim of the eyelid with the sterile cotton swab.
Place the swab immediately in a bacterial transport medium or, if the specimen is brought to the laboratory immediately, in a sterile test tube with 0.5 mL of phosphate buffered saline.
Direct Immuno-fluorescence (DIF) Antibody labeling for
With Giema stain
With Iodine stain
Giemsa stain of Chlamydia inclusion bodies (purple "caps" on epithelial cell).
Any Questions ?