Molecular Microbial Ecology Group        Department of Microbiology

Molecular Microbial Ecology Group Department of Microbiology PowerPoint PPT Presentation


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Molecular Microbial Ecology Group Department of Microbiology

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1. Molecular Microbial Ecology Group Department of Microbiology 1

2. Molecular Microbial Ecology Group Department of Microbiology 2 Mercury Cycle in the Biosphere

3. Molecular Microbial Ecology Group Department of Microbiology 3 Mercury contaminated soils

4. Molecular Microbial Ecology Group Department of Microbiology 4 Contaminated subsurface soil

5. Molecular Microbial Ecology Group Department of Microbiology 5

6. Molecular Microbial Ecology Group Department of Microbiology 6

7. Molecular Microbial Ecology Group Department of Microbiology 7

8. Molecular Microbial Ecology Group Department of Microbiology 8 Adaptation Test

9. Molecular Microbial Ecology Group Department of Microbiology 9

10. Molecular Microbial Ecology Group Department of Microbiology 10

11. Molecular Microbial Ecology Group Department of Microbiology 11 Exogenic plasmid isolation 12 replicate isolations experiments from each soil with E.coli and P.putida as recipient bacteria

12. Molecular Microbial Ecology Group Department of Microbiology 12 Exogenous plasmid isolation

13. Molecular Microbial Ecology Group Department of Microbiology 13 The merA gene encodes a mercuric reductase that reduces Hg++ to volatile Hg(0). MerP and MerT are involved in the transportation of Hg++ to MerA in the cytosol. The merR gene encodes a Hg responsive regulator.

14. Molecular Microbial Ecology Group Department of Microbiology 14 Prokaryotic mercuric reductase protein diversity

15. Molecular Microbial Ecology Group Department of Microbiology 15 16S rDNA clone library

16. Molecular Microbial Ecology Group Department of Microbiology 16

17. Molecular Microbial Ecology Group Department of Microbiology 17

18. Molecular Microbial Ecology Group Department of Microbiology 18 Cultivation of Hg++ resistant subsurface bacteria Microcultivation approach that simulates the natural growth conditions See our poster tonight! Use dilute media as proposed by Janssen et al. (AEM 2002) and long-term incubation (6-12 weeks)

19. Molecular Microbial Ecology Group Department of Microbiology 19

20. Molecular Microbial Ecology Group Department of Microbiology 20

21. Molecular Microbial Ecology Group Department of Microbiology 21 Direct detection of HGT

22. Molecular Microbial Ecology Group Department of Microbiology 22 Flow cytometry

23. Molecular Microbial Ecology Group Department of Microbiology 23 Direct detection of HGT Rhizosphere 12-38% 100-1000

24. Molecular Microbial Ecology Group Department of Microbiology 24 Sorting transconjugant bacteria for molecular analysis

25. Molecular Microbial Ecology Group Department of Microbiology 25 Conclusions We have isolated several Hg++ resistance plasmid from subsurface bacteria. We can culture hitherto unculturable Hg++ tolerant subsurface bacteria. We have a new method for detecting plasmid transfer between subsurface bacteria without cultivation. We have developed DNA-microarray for characterization of plasmids.

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