Glucose catabolism
Download
1 / 11

Glucose Catabolism - PowerPoint PPT Presentation


  • 120 Views
  • Uploaded on

Glucose Catabolism. Carbohydrate Metabolism. We convert all ingested carbs into glucose The breakdown of glucose generates ATP (energy) Anaerobic respiration (without oxygen) Fewer ATP Aerobic respiration (with oxygen) More ATP. This weeks activities:.

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about ' Glucose Catabolism' - kieran-silva


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript

Carbohydrate metabolism
Carbohydrate Metabolism

  • We convert all ingested carbs into glucose

  • The breakdown of glucose generates ATP (energy)

  • Anaerobic respiration (without oxygen)

    • Fewer ATP

  • Aerobic respiration (with oxygen)

    • More ATP


This weeks activities
This weeks activities:

  • Demonstration of heat energy from cellular respiration in plants

  • CO2 production from yeast fermentation

  • Reduction reaction in one step of Kreb’s Cycle

  • Oxygen consumption during cellular respiration in meal worms


Alcoholic fermentation by yeast
Alcoholic Fermentation by Yeast

Glucose  Ethanol + CO2 + ATP (Energy)

Tube A Tube B Tube C

10 ml yeast 10 ml yeast 10 ml yeast

H20 to fill glucose to fill 10 ml NaF

glucose to fill


Fermentation expected results
Fermentation Expected Results

How can you tell if fermentation occurs? (i.e. what is being measured?)

In which tubes do you expect fermentation to occur? Why?

Tube A Tube B Tube C

10 ml yeast 10 ml yeast 10 ml yeast

H20 to fill glucose to fill 10 ml NaF

glucose to fill


Kreb s cycle
Kreb’s cycle


Experimental reaction using ground meat
Experimental Reaction Using Ground Meat

Hydrogen/Electron accepter

Methylene Blue + H2 (colorless/pink meat)

Methylene Blue (dark blue)

Succinate

Fumarate

Succinate dehydrogenase

Substrate

Product

Enzyme

(in meat)


Kreb s set up
Kreb’s Set-Up

Tube #1 Tube #2 Tube #3 Tube #4

meat soln meat soln meat soln 5 drops s. acid

5 drops s. acid 5 drops s. acid 3 drops m. blue 3 drops m. blue

3 drops m. blue 3 drops m. blue

10ml NaF

  • 37 °C Water Bath

  • Observe until tube #1’s color changes from blue to clear

  • Remove all tubes, observe & record

  • Add water until half full all “meat” tubes, vortex & empty into bag

  • Clean tubes at sink with soap & water; rinse well



Mealworm respiration
Mealworm Respiration darkling beetle)

  • Measure distance Methylene blue drops moves in two tubes (in cm)

  • O2 consumed = difference X 0.02cm2

  • Weight of all three worms in grams (1st)

  • O2 consumed/gm (divide O2 consumed by weight)

  • Resp rate = O2 consumed ÷ 0.17 hr

    gm


Quiz 7 study guide
Quiz 7 study guide darkling beetle)

  • Understand the set-up and results from each experiment:

    • yeast alcoholic fermentation

    • succinate Kreb’s cycle

    • the mealworm respiration (oxygen consumption)

    • plant respiration demo

  • Read lab 8: Transporting Molecules


ad