Glucose catabolism
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Glucose Catabolism. Carbohydrate Metabolism. We convert all ingested carbs into glucose The breakdown of glucose generates ATP (energy) Anaerobic respiration (without oxygen) Fewer ATP Aerobic respiration (with oxygen) More ATP. This weeks activities:.

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Glucose Catabolism

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Glucose catabolism

Glucose Catabolism


Carbohydrate metabolism

Carbohydrate Metabolism

  • We convert all ingested carbs into glucose

  • The breakdown of glucose generates ATP (energy)

  • Anaerobic respiration (without oxygen)

    • Fewer ATP

  • Aerobic respiration (with oxygen)

    • More ATP


This weeks activities

This weeks activities:

  • Demonstration of heat energy from cellular respiration in plants

  • CO2 production from yeast fermentation

  • Reduction reaction in one step of Kreb’s Cycle

  • Oxygen consumption during cellular respiration in meal worms


Alcoholic fermentation by yeast

Alcoholic Fermentation by Yeast

Glucose  Ethanol + CO2 + ATP (Energy)

Tube ATube BTube C

10 ml yeast10 ml yeast10 ml yeast

H20 to fillglucose to fill10 ml NaF

glucose to fill


Fermentation expected results

Fermentation Expected Results

How can you tell if fermentation occurs? (i.e. what is being measured?)

In which tubes do you expect fermentation to occur? Why?

Tube ATube BTube C

10 ml yeast10 ml yeast10 ml yeast

H20 to fillglucose to fill10 ml NaF

glucose to fill


Kreb s cycle

Kreb’s cycle


Experimental reaction using ground meat

Experimental Reaction Using Ground Meat

Hydrogen/Electron accepter

Methylene Blue + H2 (colorless/pink meat)

Methylene Blue (dark blue)

Succinate

Fumarate

Succinate dehydrogenase

Substrate

Product

Enzyme

(in meat)


Kreb s set up

Kreb’s Set-Up

Tube #1 Tube #2 Tube #3 Tube #4

meat solnmeat soln meat soln 5 drops s. acid

5 drops s. acid5 drops s. acid 3 drops m. blue 3 drops m. blue

3 drops m. blue3 drops m. blue

10ml NaF

  • 37 °C Water Bath

  • Observe until tube #1’s color changes from blue to clear

  • Remove all tubes, observe & record

  • Add water until half full all “meat” tubes, vortex & empty into bag

  • Clean tubes at sink with soap & water; rinse well


Using a living organism mealworm larva stage of the darkling beetle

Using a living organism: mealworm (larva stage of the darkling beetle)


Mealworm respiration

Mealworm Respiration

  • Measure distance Methylene blue drops moves in two tubes (in cm)

  • O2 consumed = difference X 0.02cm2

  • Weight of all three worms in grams (1st)

  • O2 consumed/gm (divide O2 consumed by weight)

  • Resp rate = O2 consumed ÷ 0.17 hr

    gm


Quiz 7 study guide

Quiz 7 study guide

  • Understand the set-up and results from each experiment:

    • yeast alcoholic fermentation

    • succinate Kreb’s cycle

    • the mealworm respiration (oxygen consumption)

    • plant respiration demo

  • Read lab 8: Transporting Molecules


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