PROJECT PROFILE
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PROJECT PROFILE. Title : “Referral Centre for Virus Testing of Tissue Culture Raised Plants” Duration : Five years Date of Start : 28 th September 2006

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PROJECT PROFILE

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Project profile

PROJECT PROFILE

Title : “Referral Centre for Virus Testing of Tissue Culture Raised Plants”

Duration : Five years

Date of Start : 28th September 2006

Date of Termination : 27th September 2011

Project Cost (Rs.) : 75.05 Lakhs

Project Personnels

Principal Investigator: R.K. Jain

Co-Investigators : Padma Ramachandran/

V.K. Baranwal / Bikash Mandal

Research Associates : Sudeep Bag / Surekha Agarwal


Project profile

RESEARCH APPROACH

  • Confirmation of results on diagnosis of diagnostic test centers, whenever required

  • Development of diagnostic probes/kits for viruses

  • Development, standardization and validation of virus test protocols

  • Training of personnel of diagnostic centers

  • Maintenance of reference virus cultures and diagnostic reagents

SERVICE APPROACH

Major Objectives


Project profile

SERVICE APPROACH

Prepared write-up on Standard Operating Procedures (SOPs) for virus testing of tissue culture raised plants to be followed by Accrediated Test Laboratories (ATLs)


Project profile

SERVICE APPROACH

Imparted training to personnel from Accredited Test Laboratories and other Institutions

ATLs Technical undergoing training


Project profile

SERVICE APPROACH

Supplied Diagnostic Reagent to various laboratories for virus detection


Project profile

Service Approach

Testing of plant samples for freedom from viruses


Project profile

SERVICE APPROACH

  • Resource generation through testing of samples for freedom from viruses and other pathogens

    • Tissue culture industries

    • National Plant Quarantine Station

Rs. 1,40,000/-


Project profile

RESEARCH & DEVELOPMENT APPROACH

Developed polyclonal antiserum to coat protein of PRSV using recombinant technology

M 1 2 3 4

M 1 2

Amplification by RT-PCR

Cloning in pGEMT Easy Vector

~850 bp

~850 bp

Amplification of coat protein gene, Lane 1: helathy; Lane 2: infected sample

PRSV infected leaf

Mobilization of coat protein gene into expression vector (pMAL-C2X)

M 1 2 3 4 5 6

Purification of expressed coat protein

Production of polyclonal antiserum

~850 bp


Project profile

Detection of Papaya ring spot virus (PRSV) in infected papaya and other hosts samples collected from different geographical locations by direct antigen coated enzyme linked immunosorbent assay (DAC-ELISA)


Project profile

RESEARCH & DEVELOPMENT APPROACH

Developed polyclonal antiserum to coat protein of CTV using recombinant technology

M 1 2 3 4

CTV infected material

M 1 2 3 4 5 6 7 8 9

~700 bp

Cloning in pGEMT Easy

Amplification by RT-PCR

~700 bp

Mobilization of coat protein gene into expression vector (pMAL-C2X)

M S IS FT W F4 F5 F8 F11MBP

M S IS FT W F4 F5 F8 F11MBP

Purification of expressed coat protein

M 1 2 3 4 5 6 7

~66 Kd

~66 Kd

~700 bp

50 Kd

50 Kd

M : Marker; S: Soluble fraction; IS: Insoluble fraction; FT: Flowthrough; W: Wash; F4-11: eluted protein fraction

Validation of Antiserum at IIHR, Bangalore and IARI, Regional Station, Kalimpong


Project profile

Developed repository of diagnostic reagents for virus diagnosis


Acknowledgements

Acknowledgements

  • Department of Biotechnology DBT, New Delhi

  • Indian Agricultural Research Institute (IARI) , New Delhi

  • Research Associates- Sudeep Bag and Surekha Agarwal


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