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Ex. 22 Enzymatic Attacks on Nitrogen Containing Substrates

Ex. 22 Enzymatic Attacks on Nitrogen Containing Substrates. Enzymes that attack “N” substrates. 1. Urease 2. Gelatinase 3. Nitrate Reductase. Urease Urea + water ammonia + carbon dioxide Urea Broth Urea pH Indicator Acidic = Yellow Basic = Pink.

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Ex. 22 Enzymatic Attacks on Nitrogen Containing Substrates

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  1. Ex. 22 Enzymatic Attacks on Nitrogen Containing Substrates

  2. Enzymes that attack “N” substrates • 1. Urease • 2. Gelatinase • 3. Nitrate Reductase

  3. Urease Urea + water ammonia + carbon dioxide Urea Broth Urea pH Indicator Acidic = Yellow Basic = Pink

  4. UreaseTest

  5. Gelatinase Gelatin (solid) Gelatin (liquid) Tryptic Soy Gelatin Agar

  6. Gelatinase Test

  7. Nitrate Reductase Nitrate Nitrite + water Facultative Bacteria Nitrate Broth

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