Detection of single red blood cell magnetic property using a highly sensitive gmr sv biosensor
Download
1 / 26

Detection of Single Red Blood Cell Magnetic Property using a Highly Sensitive GMR-SV Biosensor - PowerPoint PPT Presentation


  • 101 Views
  • Uploaded on

Detection of Single Red Blood Cell Magnetic Property using a Highly Sensitive GMR-SV Biosensor. Sang-Suk Lee, Sang-Hyun Park Kwang-Suo Soh 2006.9.27 CKC Symposium. Contents. Magnetism and Sensitivity New Functional Soft Magnetic Materials Measurement and Resolution

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about ' Detection of Single Red Blood Cell Magnetic Property using a Highly Sensitive GMR-SV Biosensor' - kesia


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
Detection of single red blood cell magnetic property using a highly sensitive gmr sv biosensor

Detection of Single Red Blood Cell Magnetic Property using a Highly Sensitive GMR-SV Biosensor

Sang-Suk Lee, Sang-Hyun Park

Kwang-Suo Soh

2006.9.27 CKC Symposium


Contents
Contents a Highly Sensitive GMR-SV Biosensor

  • Magnetism and Sensitivity

    • New Functional Soft Magnetic Materials

    • Measurement and Resolution

  • Red Blood Cell Magnetophrosis

    • Oxygen - RBC

    • Magnetic Susceptibility

    • Magnetophoretic Mobilities

  • Set up of Measurement System

    • Micro Capillary Technology

    • Optical Tweezer Technology

  • Further Corporation Environment

    • Research Field of Prof. Tony Bland’s Group

    • Future Research Plans


Metals spin polarization p and magnetism
Metals, Spin polarization a Highly Sensitive GMR-SV Biosensor(P), and Magnetism

Metal : n() = n() ( P = (n()-n())/(n()+ n()) =0 )

Ferromagnetism : ( 0<P <1 )

Half Metals: CrO2, Fe3O4, PtMnSb (P = 1)

3d 10-x

4f 14-x


Four general types of a magnetism
Four general types of a Highly Sensitive GMR-SV Biosensora magnetism


Properties of GMR-SV Multilayers

Ta 5 nm

Hc

FM (Free Layer)

NiFe 10 nm

NFM (Spacer)

Cu 2.6 nm

FM (Pinned Layer)

NiFe 4.0 nm

AFM (Pinning Layer)

  • M-H curve

FeMn 7.0 nm

Ta 5 nm

Rap

Sensing

position

Rp

Rp

  • M-R curve

MR Ratio  (Rap-Rp)/Rp = 4 ~ 9 %

Magneto sensitivity MR/H


Advantage of GMR-SV Biosensor Multilayers

  • The low requirement for sample amount

  • Easy integration for multianalyte detection on a single chip

  • Inexpensive and portable devices requiring little or no expertise

  • for their use

Application of GMR-SV Biosensor

Silica coated magnetic nanoparticles

Replace by RBC

PR(1.3 um)

SiO2(100 nm)

Contact pad (160 nm)

SV Sensor

Silicon substrate


Highly sensitive magnetic films
Highly Sensitive Magnetic Films Multilayers

  • Ni77Fe14Cu5Mo4

    (Conetic film (Mu-metal))

  • Optimized condition : Hc = 0.055 Oe

  • Minimized purpose : ~0.055 Oe

    (predicted values)

    MS(MR/H) = 50 ~150 %/Oe

  • One of several hundreds for Hc of NiFe

    Hc = 5~10 Oe

    MS(MR/H) = 0.5 ~1.5 %/Oe

  • Measurement by using SQUID

    • Sensitivity - nano tesla (10-9 T)

      => 10-5 Oe

    • NiFe, NiFeCo => 10-2~10-3 Oe

    • NiFeCuMo => 10-4~10-5 Oe

      (theoretically 10-6)


Expectation of a Very High Sensitivity of GMR-SV Multilayers

Earth field

Electric Instruments

around field

Cosmos Magnetic field

High Volt

Transmitter,

Transformer,

Choke Coil,

Motor

Bio-magneto signal

General & Super-

Conductor Magnet

Magnetic field measuring limit

EEG

ECG

Permanent

Magnet

Tesla

Sensitivity of GMR/SV Biosensor

  • Sensor size : 26 m2

  • Output : 100 V , Resolution : 100 nT = 10-3 G

  • M = 510-22 emu (erg/G)  5 10-2 B


The Hemoglobin Properties Multilayers

Of Red Blood Cell

deoxyhemoglobin

methemoglobin

oxyhemoglobin

  • Ferrous iron(Fe2+)

  • Fe2O3

  • Binding Oxygen

  • Molecules

  • 2-pair Polypetide Chain

  • Globin+4 Heme Group

  • Ferric iron(Fe3+)

  • Fe3O4

  • Loss of carrier power of oxygen and carbon dioxide

  • Blue-green color

* RBC : normal adult blood volume = 46 L

average number = 45×106/cc

circulatory lifetime = 120 days

1 RBC = 3×106 Hemoglobin

1 Hemoglobin = 4 Fe atoms


Ligand & Light Absorption Hemoglobin and Fe Multilayers

 Diamagnetic Properties

 Paramagnetic Properties


Red Blood Cell Multilayers

Magnetophoresis-1

1.Capillary magnetophoresis of Human blood cells

trapping in a flow systemJ. of Chromatography A, 2002

Apparatus

Results


Red Blood Cell Multilayers

Magnetophoresis-2

2. Red Blood Cell Magnetophrosis

Maciej Zborowski et al, Biophysical Journal 84, 2638 (2003)

  • The measured magnetic moments of hemoglobin :

  • its compounds on the relatively high hemoglobin concentration of human erythrocytes

  • 2) Differential migration of these cells was possible

  • if exposed to a high magnetic field (1.40 T).

  • 3) Development of a new technology, cell tracking velocimetry (CTV) the migration velocity of oxy-, deoxy-, and metHb-containing erythrocytes


Red Blood Cell Multilayers

Magnetic Susceptibilities


Red Blood Cell Multilayers

Magnetophoretic Mobilities


Detection of Magnetic Nanoparticles Multilayers

Ring Pattern by Liquid Drop Motion of Nano-particles

Before drop

After drop :

formation of

ring pattern


Output Sensing Signal Observation of Nanopartices Multilayers

Change of Sensing Position by the abrupt Variationof Magnetic Field

Drop point

Before state : max & min signal


Capillary Capture Multilayers

Red Blood Cell

Biophysics of cell membranes :

Investigation of the changes in the mechanical and

rheological properties of blood cells in diabetes

Taken by http://newton.ex.ac.uk/research/biomedical/membranes/





Micro-hole Capillary with RBC Infrared

and Biosensor

Pure-RBC

26 m2


Capillary and Approach to Biosenor Infrared

GMR-SV Biosensor

Red Blood Cells

Capillary

Red Blood Cell


Micro-capillary Moving Infrared

and Manipulating Images

Needs and supplememts:

Advanced Microscope, CCD Images, Uptaking RBC Techniques


Biological Cell Detection using Infrared

Ferromagnetic Microbeads {by T. Bland’ Group}


Integrated microfluidic cell with multilayer ring sensors for single magnetic microbead detection {by T. Bland’ Group}


Future Research Plans for single magnetic microbead detection {by T. Bland

To obtain an analytic value of bio-magnetic molecules

such as : RBC, Hemo-Sanal, etc

Using : (1) Micro-capillary controlling technology

(2) Optical tweezer trapping and manipulation

Fabrication of high sensitive GMR/SV biosensor

Fabrication of a highly sensitive GMR/SV biosensor with conetic film

Extraction of RBC or Hemo-Sanal from Bonghan Duct

Extraction of RBC or Heme-Sanal from Bonghan Duct

Investigation of single RBC’s and

Hemo-Sanal’s magneto-properties

< Dec. 2006

 Feb. 2007 >

BPL, SNU,

CKC Research

< Dec. 2006

 Feb. 2007 >

Nano-bio Lab.

Sangji University

< Sept. 2006

 Nov. 2007 >

Practical use of biosensor and

medical instruments

Set up measuring system,

using micro-capillary and optical tweezer


ad