Sustainable production and utilization of helo as an edible berry and ornamental crop
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Sustainable Production and Utilization of ‘ Ōhelo as an Edible Berry and Ornamental Crop

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Sustainable production and utilization of helo as an edible berry and ornamental crop

Sustainable Production and Utilization of ‘Ōheloas an Edible Berry and Ornamental Crop

Francis Zee1, Lisa Keith1, Amy Strauss1, Claire Arakawa1, Tristan Foote1, Kim Hummer2, Barbara Reed2, Nahla Bassil2, Stuart T. Nakamoto3, Randall Hamasaki3, Milton Yamasaki3, Andrew Kawabata3, Bob Durst4, Allan K. Ikawa5, Jodi Silva5, Ken Love6, Yongjian Chang7


Scri project goals

SCRI Project Goals

  • Clonal propagation and production protocols

  • Establish molecular fingerprinting and

  • Cryopreservation methodology

  • Conduct fruit nutrient analyses

  • Determine host-pathogen interactions


Scri project goals1

SCRI Project Goals

  • Produce ohelo as an ornamental from flask to nursery to market

  • Prepare economic feasibility analyses of ohelo as ornamental potted plant

  • Provide extension and outreach

  • Develop seed based production protocols

  • Initiate value added products and culinary research


P l goddess of fire

Pélé Goddess of Fire

  • Destroyed >100 structures since 1983

  • Added >70 acres to the SE coast


Sustainable production and utilization of helo as an edible berry and ornamental crop

Ōhelo


Attractive red and green foliage

Attractive red and green foliage


Sustainable production and utilization of helo as an edible berry and ornamental crop

Attractive flowers


Sustainable production and utilization of helo as an edible berry and ornamental crop

Cultivated for edible fruits


Helo plants around the volcano area

‘Ōhelo plants around the Volcano Area


Goals

Goals

  • Reduce wild gathering of ‘ōhelo for commercial uses.

  • Reduce traffic and the spread of invasive weeds

  • Provide a sustainable (supply) of berries for culinary and value added product research (demand).

Two hand-full of berries to start


Sustainable production and utilization of helo as an edible berry and ornamental crop

Dr. Francis Zee and Vaccinium reticulatum seedlings


Ohelo cultivar releases 2009

Ohelo Cultivar Releases 2009

  • Kilauea

  • Nene

  • Red Button


New selections

New Selections


Sustainable production and utilization of helo as an edible berry and ornamental crop

‘Ōhelo

Propagation

Seed, Cuttings, Tissue Culture

Identification

Utilization

Fruit nutrient analysis

Culinary uses


Sustainable production and utilization of helo as an edible berry and ornamental crop

‘Ōhelo

  • PROPAGATION

    • Seed

    • Cuttings

    • Tissue Culture

      Cold storage/ Cryogenics


Ohelo from seeds

Ohelo from seeds …


To seed

… to seed

Ohelo seeds


Helo seed storage and germination

‘Ōhelo seed storage and germination

Barbara Reed USDA-ARS


Clonal propagation of helo

Clonal Propagation of ‘ōhelo


Cold storage in semipermeable bags for 12 16 months

Cold Storage in semipermeable bagsfor 12-16 months

Red Button

Nene

Kilauea

Barbara Reed USDA-ARS


Cold storage of shoot cultures

Cold Storage of Shoot Cultures

Barbara Reed USDA-ARS


Sustainable production and utilization of helo as an edible berry and ornamental crop

‘Red Button’ cryopreserved with encapsulation dehydration. S = sucrose treatment, D = dehydration, the others were exposed to liquid nitrogen

Barbara Reed USDA-ARS


Cryopreservation of helo clones

Cryopreservation of ‘ōhelo clones

Barbara Reed USDA-ARS


Sustainable production and utilization of helo as an edible berry and ornamental crop

Field and Nursery management

Produced 50 lb of ‘ōhelo berry for R&D

May to July 2010

17.2 lbs from field planting

33.1 lbs from potted ohelo plants

NO9-16 clones


Sustainable production and utilization of helo as an edible berry and ornamental crop

A sustainable source of ‘ōhelo seeds for germplasm requests


Sustainable production and utilization of helo as an edible berry and ornamental crop

USDA/ARS display and information table

Six inch ohelo seedlings for BIAN plant sale

NO6-7 Bonsai


Sustainable production and utilization of helo as an edible berry and ornamental crop

‘Ōhelo

  • Holiday foliage plant

  • Landscape plant

  • Fruit production

  • Culinary product development


Sustainable production and utilization of helo as an edible berry and ornamental crop

Ōhelo


Sustainable production and utilization of helo as an edible berry and ornamental crop

‘Ōhelo

  • Identification by Molecular Markers

    • Cultivar –

    • Genetic Diversity assessment


Sustainable production and utilization of helo as an edible berry and ornamental crop

Microsatellite marker-based fingerprints for three important selections

Red Button

Kilaua

Nene

294

294

298

294

296

308

  • Tested 23 blueberry (V. corymbosum) SSRs

  • NA741: an example of one SSR that differentiates between 3 selections


Sustainable production and utilization of helo as an edible berry and ornamental crop

Microsatellite marker-based fingerprints for three important selections

Eight SSRs reliably distinguish between these selections


Sustainable production and utilization of helo as an edible berry and ornamental crop

Dendrogram based on microsatellite

analysis using six primer pairs.


Sustainable production and utilization of helo as an edible berry and ornamental crop

83

V ret Ohelo 6

V ret Ohelo 9

V ret 780

Hawaiian Taxa

V ret Ohelo 7

71

V cal 1812

V cal 1814

96

V praes 1626

97

V. praestans

V praes 1569

V praes 1570

V memb 1372

99

V memb 141

V memb 1213

V scop

V myrt 464

V myrt 1622

V myrt 379

V oval 1118

V oval 1189

V parv 436

V myrt 627

V oval 1421

V cesp 1489

Bluecrop

V cesp 1651

V oval 1546

100

V myrt 1684-1

V myrt 1684-3

V oval 1375

V del 403 3

V del 403 6

V oval 1252

V oval 1258

0.05

Section Myrtillus

  • SSRs for 10 species in section Myrtillus

  • Hawaiian taxa more recently derived

  • Russian V. praestansgrouped together

  • Genetically identical V. myrtillus 1684-1 and 1684-3 from Kalmiopsis Wilderness, OR.


Sustainable production and utilization of helo as an edible berry and ornamental crop

  • Germplasm conservation

  • Sustainable germplasm resource for

  • research

  • Ornamental potted plants

  • Alternate source from wild harvest

  • Sustainable and reliable source of berry for culinary and value added product uses.


Diseases of helo

Diseases of ‘Ōhelo

Pucciniastrumvaccini

Rust

Leaf spots and sudden collapse - Calonectria sp.

PowderyMildew

Microsphaera sp.

30 days after inoculation


Fruit analysis summary

  • Anthocyanins

    • Similar to cranberry, but v. low peonidin

  • Rich source of phenolics

  • Acids

    • Similar profile to cranberry, but lower amounts

  • Sugar profile

  • Extremely Good source of PAC’s

    • Especially V. calycinum

Fruit Analysis Summary


Pro anthocyanadins

Pro Anthocyanadins

10 x as much!


Helo utilization

‘Ōhelo - Utilization

Culinary recipes

  • Ohelo Berry and Horseradish Pot Roast

  • SweettartOhelo-Berry Compote

  • Foie Gras with Ohelo Berries

  • Goat Cheese and Ohelo Berry Bruschetta

  • Grilled Lime Tequila Chicken with Ohelo Berry and Pineapple Sauce

  • Ohelo and Oatmeal Pie

  • Ohelo glazed Duck

  • Ohelo creamed cheese pie


Scri project goals2

SCRI Project Goals

  • Clonal propagation and production protocols

  • Establish molecular fingerprinting

  • Cryopreservation methodology

  • Conduct fruit nutrient analyses

  • Determine host-pathogen interactions


Scri project goals3

SCRI Project Goals

  • Produce ohelo as an ornamental from flask to nursery to market

  • Prepare economic feasibility analyses of ohelo as ornamental potted plant

  • Provide extension and outreach

  • Develop seed based production protocols

  • Initiate value added products and culinary research

SUCCESS!


Cooperators

Cooperators

1U.S. Dept. Agriculture, Agricultural Research Service (USDA, ARS) Pacific Basin Agricultural Research Center (PBARC) P.O. Box 4487, Hilo, Hawaii 96720;

2USDA ARS National ClonalGermplasm Repository (NCGR), 33447 Peoria Road, Corvallis, Oregon, 97333-2521;

3University of Hawaii, College of Tropical Agriculture and Human Resources (UH, CTAHR), 1955East-West Rd. AgSci 314B, .Honolulu, HI 96822;

4Linus Pauling Institute, Oregon State University, Corvallis, OR

97331-6512;

5Research & Development, Big Island Candies, Hilo, HI;

6Hawaii Tropical Fruit Grower Association, President and Agriculture Chair of the American Culinary Federation chapter – Kona Kohala Chefs Association, Kona, HI;

7North American Plants LLC, McMinnville, OR 97128-8410.


Sustainable production and utilization of helo as an edible berry and ornamental crop

Thank you to everyone!


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