igem @ imperial
Download
Skip this Video
Download Presentation
iGEM @ Imperial

Loading in 2 Seconds...

play fullscreen
1 / 23

iGEM @ Imperial - PowerPoint PPT Presentation


  • 99 Views
  • Uploaded on

iGEM @ Imperial. Week 1 Engineering/Biology Introduction Lectures Journal Club Wiki Brainstorming 3 ideas. Starting off: Week 1. Only three ideas made the cut. Bio-Clock (Re-defining Time). Pulse of AHL moves along a gutter of medium Cells fluoresce when activated Refractory period

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about ' iGEM @ Imperial' - kermit-baxter


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
starting off week 1
Week 1

Engineering/Biology Introduction Lectures

Journal Club

Wiki

Brainstorming

3 ideas

Starting off: Week 1
bio clock re defining time
Bio-Clock (Re-defining Time)
  • Pulse of AHL moves along a gutter of medium
  • Cells fluoresce when activated
  • Refractory period
  • The time period is controlled by
    • the radius
    • gutter width
    • cell density
bio memory
Bio-Memory

1010100000000011111000010101011111100000000111111111000001010100010100001000010100101001010000001001010101001010101001101010101001010

  • Data stored in bacteria written using a green laser
  • Data read using a UV laser + fluorimeter
  • Cells either 1 (RFP) or 0 (no RFP)
  • Data stored in switch

Very High Compression due to small size of bacteria

the oscillator
The Oscillator
  • Culture Wide oscillations of AHL
  • Frequency must be tuned easily
  • Oscillations must be stable

Changing Concentration of AHL

slide8
Week 2

Investigation of all 3 ideas

Modelling

Evaluating risks

Start work in the Wet lab

Decision on the Oscillator as main project;can use other ideas as further developments

Week 3

Further research

Modelling

Assembly of parts

Protocols for testing parts

Setting up OWW

predator prey dynamics
Predator Prey Dynamics

Simply Make a Bio-chemical system that can do this.

Lotka-Voltarra Model output

design

A

A

B

B

Design
  • Positive Feedback of A
  • AB Induces production of more B
  • Both A and B are used to make AB
two cell system

A

B

Two Cell System
  • Two independent populations of Cells
  • These cells do not kill each other
  • Altering the initial ratios of these cells will alter the frequency of oscillations
design cell1 prey

A

A

A

A

Design Cell1 (Prey)
  • Prey cell must produce molecule A exponentially

Lux R is produced which detects molecule A

Pc

Lux R

Then initiates transcription at Plux

Pc is always on

Lux I

Plux

Which Produces More A

design1
Design

The Predator Cell

The role of a predator is to reduce the prey numbers as a function of the predator population numbers.

Predator

Detect Prey Population Size

Reduce Prey Population Size

design2

Lux R

aiiA

Plux

Design

The Predator Cell

Detects Prey Population Size

Reduces Prey Population Size

design3
Design

The Predator Cell

A

LuxR

A

A

LuxR

aiiA

Lux R

aiiA

Plux

design entire system

Pc

Lux R

Lux I

Plux

A

A

A

Design (Entire System)

Diffusion

Extra cellular pool of A (HSL)

(this should oscillate)

The Prey Cell

The Predator Cell

LuxR

A

A

LuxR

aiiA

Lux R

aiiA

Plux

modelling
Modelling
  • Tom’s Monster
a shocking discovery at first sight
A shocking discovery: at first sight...
  • After finishing our oscillator design...
  • MIT Project 2004:
      • Cell-Cell synchronized Oscillator Design
      • Similar approach using concepts of quorum sensing
      • BUT: This system does not use predator-prey dynamics and is implemented in a single cell (ours is multicellular

MIT Oscillator Design

http://web.mit.edu/~cbatten/www/work/ssbc04/system-spec-ssbc04.pdf

communication the wiki
Communication: The Wiki
  • Wiki-Newspaper
  • Documentation for future references
  • Communication
    • Within the team
    • With other teams
  • Monitoring progress (Gantt Chart)
  • Present ourselves & our project
outline
Outline
  • Further modelling & testing of parts
  • Parts assembly
  • Phase 2
    • Coupling the oscillator to a biological to electrical interface
    • Synchronizing oscillations 2 petri dishes
ad