Real World Project: Using HPLC and SFE to determine amounts of caffeine in different coffee samples. Danielle Tague Khadija Mitchell. Purpose. Determination of the amount of caffeine in decaf and caffeinated ground coffee and coffee beans was done using HPLC.
Danielle TagueKhadija Mitchell
Determination of the amount of caffeine in decaf and caffeinated ground coffee and coffee beans was done using HPLC.
Extraction of caffeine from each sample was done by SFE.
The amounts of caffeine determined using HPLC were compared with the amounts of caffeine extracted using SFE.
Caffeinated samples were higher as expected
Decaff was not as low as expected
Bean samples were not reliable because they didn’t dissolve completely – but caffeinated beans still had higher results
Extracted caffeine was also useless because it did not dissolve and results were inconclusive
The left over samples from SFE should have been lower since the caffeine was extracted from them
Our extracted caffeine should have been dissolved in either methanol or chloroform rather than water
We did not initially do this to be consistent with other samples
We could have reran the samples through the SFE again to attempt to extract more but we ran out of time