Real world project using hplc and sfe to determine amounts of caffeine in different coffee samples
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Real World Project: Using HPLC and SFE to determine amounts of caffeine in different coffee samples. Danielle Tague Khadija Mitchell. Purpose. Determination of the amount of caffeine in decaf and caffeinated ground coffee and coffee beans was done using HPLC.

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Real world project using hplc and sfe to determine amounts of caffeine in different coffee samples

Real World Project:Using HPLC and SFE to determine amounts of caffeine in different coffee samples

Danielle TagueKhadija Mitchell


Purpose

Purpose

Determination of the amount of caffeine in decaf and caffeinated ground coffee and coffee beans was done using HPLC.

Extraction of caffeine from each sample was done by SFE.

The amounts of caffeine determined using HPLC were compared with the amounts of caffeine extracted using SFE.


Data sfe

Data: SFE

  • We weighed the flasks before and after the extraction and took the differences to determine amounts extracted.

  • The ground caffeinated coffee sample did not yield results we would have expected – if any caffeine was extracted at all it was a very minimal amount.

  • The other samples showed results we expected, low amounts of caffeine in decaf samples and a higher amount in the caffeinated sample.


Data hplc from original samples

Data: HPLC From Original Samples

  • Dissolved 50 mg samples in 50 mL distilled water over heat.

  • Bean samples did not completely dissolve


Original samples

Original Samples

  • Concentration of caffeine in the ground decaff sample was lower than caffeinated as expected

  • Concentrations in the beans were low (due to difficulty in dissolving beans)


Data hplc from sfe

Data: HPLC From SFE

  • Left over sample from SFE, 50 mg, was dissolved in 50 mL distilled water.

  • Bean samples did not completely dissolve.

  • Caffeine extract was dissolved in 50 mL distilled water. (methanol or chloroform)


Samples from sfe

Samples from SFE

  • The values of the two ground samples were too close

  • Bean samples were low because it was hard to dissolve

  • Inconclusive and low data for the extracted caffeine because it was not able to bedissolved.


Conclusion of results

Conclusion of Results

Caffeinated samples were higher as expected

Decaff was not as low as expected

Bean samples were not reliable because they didn’t dissolve completely – but caffeinated beans still had higher results

Extracted caffeine was also useless because it did not dissolve and results were inconclusive

The left over samples from SFE should have been lower since the caffeine was extracted from them


Error

Error

Our extracted caffeine should have been dissolved in either methanol or chloroform rather than water

We did not initially do this to be consistent with other samples

We could have reran the samples through the SFE again to attempt to extract more but we ran out of time


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