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BONE

BONE . SSN Histology . The Basics. Specialized connective tissue: mineralized matrix (hydroxyapatite) Type I Collagen; Proteoglycans; Non-collagenous Glycoproteins Functions: Mechanical support Calcium homeostasis Encloses bone marrow. Cells.

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BONE

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  1. BONE SSN Histology

  2. The Basics • Specialized connective tissue: • mineralized matrix (hydroxyapatite) • Type I Collagen; Proteoglycans; Non-collagenous Glycoproteins • Functions: • Mechanical support • Calcium homeostasis • Encloses bone marrow

  3. Cells Osteoblasts = osteocyte progenitors; secrete ground substance for osteoid Lab 5, Slide 30

  4. Cells Osteocytes = mature, non-dividing bone cells; enclosed in calcified bone matrix

  5. Cells Osteoclasts = remodeling/ resorption by using lysosomal enzymes

  6. Cells Osteoclasts: Active bone resorption Limited bone resorption

  7. Mature vs. Immature Bone Mature (Lamellar) Compact (Cortical) Spongy (Cancellous) • w/ Haversian systems • w/ trabecular appearance

  8. Mature vs. Immature Bone Immature Bone (Woven) • Nonlamellar • Found in fetus or following a fracture

  9. Appositional vs. Interstitial • Appositional = bone growth on pre-existing bone surface • Note that bone tissue only undergoes appositional • Interstitial = bone growth via new cartilage formation within pre-existing cartilage mass • Note that it is cartilage that undergoes interstitial • Long Bone Growth: • WIDTH --> Appositional (bone) • LENGTH --> Interstitial (cartilage)

  10. Endochondral vs. Intramembranous Endochondral Intramembranous

  11. Endochondral Ossification

  12. Endochondral Ossification Zone of Proliferation Zone of Reserve Cartilage

  13. Endochondral Ossification Zone of Hypertrophy Zone of Proliferation

  14. Endochondral Ossification Zone of Calcification Zone of Resorption

  15. Preparation of Bone Samples Ground Bone

  16. Preparation of Bone Samples Ground Bone

  17. Preparation of Bone Samples Decalcified Bone

  18. Final Thoughts…. • Let staining properties guide you • Note cellularity; compare size, color, shape, relative quantity of cells • Haversian = central to osteon • Volkmann’s = perpendicular • Think of bone as specialized connective tissue; always in a dynamic state • Long bones = endochondral • Parietal bones = intramembranous • Mnemonic for Endochondral: RealPlayersHave CoolRides

  19. Question 1. . . Panel A Panel B

  20. Questions 2 & 3. . . Figure A Figure B

  21. Questions 4 & 5. .. Panel B Panel A

  22. Question 6. . .

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