Caves. Caves (cont.). Caves (cont.). Soil Crusts. Purpose: To detect if there’s biological activity in soil sample by checking for color change in the ICM solution Materials: Test tube Soil Sample PBS ICM dye solution LB (food for microorganisms). Indigo-Carmine (ICM) Experiment.
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Purpose: To detect if there’s biological activity in soil sample by checking for color change in the ICM solution
ICM dye solution
LB (food for microorganisms)
Take ONE test tube per group
Collect soil sample
Soil crust/Dry soil
Soil with no life
Put a teeny tiny amount of soil sample (about 0.2g)
Mix buffer and dye
Put a teeny tiny amount of LB liquid or glucose solution into test tube with soil sample
Fill so that upto about end of the curvy part (just about 2 small drops with pipette)
Now, fill your test tube with buffer and ICM dye solution up to the very top
Fill until there’s a sort of bubble formed at the top of the test tube,, or until you can make sure there are no air bubbles
Cap, close and shake often
Watch and wait, record any time you see a color change
If it goes from blue to green/yellow/clear, there’s biological activity (there’s living micro-organisms in the soil sample)
Record date and time each time you see a color change.
NASA Spaceward Bound: Mojave 2009