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Caves. Caves (cont.). Caves (cont.). Soil Crusts. Purpose: To detect if there’s biological activity in soil sample by checking for color change in the ICM solution Materials: Test tube Soil Sample PBS ICM dye solution LB (food for microorganisms). Indigo-Carmine (ICM) Experiment.

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Presentation Transcript
indigo carmine icm experiment
Purpose: To detect if there’s biological activity in soil sample by checking for color change in the ICM solution

Materials:

Test tube

Soil Sample

PBS

ICM dye solution

LB (food for microorganisms)

Indigo-Carmine (ICM) Experiment
indigo carmine icm experiment procedure
Take ONE test tube per group

Collect soil sample

Wet soil

Soil crust/Dry soil

Soil with no life

Put a teeny tiny amount of soil sample (about 0.2g)

Mix buffer and dye

Put a teeny tiny amount of LB liquid or glucose solution into test tube with soil sample

Fill so that upto about end of the curvy part (just about 2 small drops with pipette)

Now, fill your test tube with buffer and ICM dye solution up to the very top

Fill until there’s a sort of bubble formed at the top of the test tube,, or until you can make sure there are no air bubbles

Cap, close and shake often

Watch and wait, record any time you see a color change

If it goes from blue to green/yellow/clear, there’s biological activity (there’s living micro-organisms in the soil sample)

Record date and time each time you see a color change.

Indigo-Carmine (ICM) Experiment:Procedure
the end
The End

NASA Spaceward Bound: Mojave 2009

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