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Tau Dataset Analysis. PHF tau phosphorylation and ubiquitination sites (isoform 2). P. MAEPR QEFEV MEDHAGTYGL GDR K DQGGYT MHQDQEGDTD AGLKESPLQT PTEDGSEEPG SETSDAKSTP TAEDVTAPLV DEGAPGK QAA AQPHTEIPEG TT AEEAGIGD TPSLEDEAAG HVTQAR MVSK SKDGTGSDDK KAKGADGKTK

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PHF tau phosphorylation and ubiquitination sites (isoform 2)

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MAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQT

PTEDGSEEPG SETSDAKSTPTAEDVTAPLVDEGAPGKQAA AQPHTEIPEG

TTAEEAGIGDTPSLEDEAAGHVTQARMVSK SKDGTGSDDK KAKGADGKTK

IATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPK SGDRSGYSSP

GSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPM

PDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSK CGSKDNIKHV

PGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRV

QSKIGSLDNI THVPGGGNKK IETHKLTFRE NAKAKTDHGA EIVYKSPVVS

GDTSPRHLSN VSSTGSIDMVDSPQLATLADEVSASLAKQG L

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BLUE = sequence coverage; = phosphorylation; = ubiquitination

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Ubiquitin sequence
Ubiquitin Sequence

6 11

MQIFVKTLTG KTITLEVEPS DTIENVKAKI

18

QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN

63

IQKESTLHLV LRLRGG


K6: [email protected] K11: [email protected]

K48: [email protected] K63: [email protected]

For LysC digestion, we didn’t identify any ubiquitation sites since LysC doesn’t cut on R.


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