Light intensity (µmol m
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Light intensity (µmol m -2 s -1 ). 1000. 0. AA. - +. wt. pgr5. - +. A. 3.0. AA. 2.5. - +. wt. - +. pgr5. 2.0. NPQ. 1.5. 1.0. 0.5. 0.0. 0. 5. 10. 15. 20. 25. Duration of incubation (min). B. 0.9. Control (-Cm). +Cm. 0.8. 0.7. 0.6. 0.5. F v / F m. 0.4. 0.3.

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0 0

Light intensity (µmol m-2 s-1)

1000

0

AA

-

+

wt

pgr5

-

+

A

3.0

AA

2.5

-

+

wt

-

+

pgr5

2.0

NPQ

1.5

1.0

0.5

0.0

0

5

10

15

20

25

Duration of incubation (min)

B

0.9

Control (-Cm)

+Cm

0.8

0.7

0.6

0.5

Fv/Fm

0.4

0.3

0.2

0.1

0.0

0

1

2

3

0

1

2

3

Duration of incubation in light (h)

Supplemental Figure 1. Effect of antimycin A on NPQ and photoinhibition. Leaf discs (78.5 mm2) from the wild type and the pgr5 mutant were exposed to light at 1000 µmol photons m-2 s-1 in the presence of 250 µM antimycin A (AA) or in its absence. A, Induction of NPQ. B, Leaf discs were exposed to light in the presence of 1 mM chloramphenicol (Cm) or in its absence (Control). The maximum quantum yield of PSII (Fv/Fm) was measured after dark incubation for 15 min. In A and B, the values are mean ± SD (bars) of results from three independent experiments.


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